| Literature DB >> 36161413 |
Tiziana Annese1,2, Roberto Tamma1, Domenico Ribatti3.
Abstract
Among in vivo angiogenesis assays, chick chorioallantoic membrane (CAM) assay has allowed meaningful progress in elucidating the mechanism of the vasoproliferative response to several pro- and antiangiogenic factors, thanks to its low costs, relatively easy management, and lower ethical concerns compared to other animal in vivo models.Here, we present a method to quantify angiogenesis and antiangiogenesis processes in the CAM based on the segmentation of microscopic images of blood vessels. Images captured from in vivo CAM samples can be analyzed for their vascularization with IKOSA CAM Assay to measure their total area, length, mean thickness, and the number of branching points. This chapter presents a detailed protocol to perform a CAM assay and analysis, and the IKOSA CAM Assay output is described.Entities:
Keywords: Angiogenesis; Blood vessel; Chick chorioallantoic membrane (CAM); Image analysis; In vivo; Microscopy; Quantification
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Year: 2023 PMID： 36161413 DOI： 10.1007/978-1-0716-2703-7_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745