Yiming Wang1,2,3, Xue Wang1,2,3, Yang Yang1,2,3, Qianghua Quan1,2,3, Tong Huo1,2,3, Simin Yang4, Ruijun Ju4, Quan An1,2,3,5. 1. Research and Development Department, Yunnan Baiyao Group Health Products Co, Ltd, Kunming, People's Republic of China. 2. East Asia Skin Health Research Center, Beijing, People's Republic of China. 3. Research and Development Department, REAL DermaSci & Biotech Co, Ltd, Beijing, People's Republic of China. 4. Beijing Key Laboratory of Enze Biomass Fine Chemicals, Department of Pharmaceutical Engineering, Beijing Institute of Petrochemical Technology, Beijing, People's Republic of China. 5. Research and Development Department, Yunnan Baiyao Group Shanghai Science & Technology Co, Ltd, Shanghai, People's Republic of China.
Abstract
Background: Cannabidiol (CBD) is a non-psychoactive phytocannabinoid constituent of Cannabis sativa with pain-relieving and anti-inflammatory properties. With the emphasis on natural ingredients in cosmetics, CBD has become a new cosmetic ingredient due to its ability to alleviate inflammation. However, in-depth studies that directly compare the effective mechanism and the therapeutic potential of CBD are still needed. Purpose: The aim of the present study was to investigate the anti-inflammatory effect of CBD in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and compare it to dexamethasone (DEX). Methods: RAW264.7 macrophages in the logarithmic growth phase were incubated in the presence or absence of LPS. After that, the production of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were measured. A luciferase reporter assay for nuclear factor kappa B (NF-κB) was performed, and the phosphorylation levels of the mitogen-activated protein kinase (MAPK) and NF-κB signaling pathways were measured. Results: The present study indicated that CBD had a similar anti-inflammatory effect to DEX by attenuating the LPS-induced production of NO, IL-6, and TNF-α. However, only CBD attenuated JNK phosphorylation levels, and only DEX attenuated IKK phosphorylation levels. Conclusion: These results suggested that CBD and DEX exhibit similar anti-inflammatory effects on LPS-induced RAW264.7 macrophages mainly through suppressing the MAPK and NF-κB signaling pathways, but with different intracellular mechanisms. These findings suggested that CBD may be considered a natural anti-inflammatory agent for protecting skin from immune disorders.
Background: Cannabidiol (CBD) is a non-psychoactive phytocannabinoid constituent of Cannabis sativa with pain-relieving and anti-inflammatory properties. With the emphasis on natural ingredients in cosmetics, CBD has become a new cosmetic ingredient due to its ability to alleviate inflammation. However, in-depth studies that directly compare the effective mechanism and the therapeutic potential of CBD are still needed. Purpose: The aim of the present study was to investigate the anti-inflammatory effect of CBD in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and compare it to dexamethasone (DEX). Methods: RAW264.7 macrophages in the logarithmic growth phase were incubated in the presence or absence of LPS. After that, the production of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were measured. A luciferase reporter assay for nuclear factor kappa B (NF-κB) was performed, and the phosphorylation levels of the mitogen-activated protein kinase (MAPK) and NF-κB signaling pathways were measured. Results: The present study indicated that CBD had a similar anti-inflammatory effect to DEX by attenuating the LPS-induced production of NO, IL-6, and TNF-α. However, only CBD attenuated JNK phosphorylation levels, and only DEX attenuated IKK phosphorylation levels. Conclusion: These results suggested that CBD and DEX exhibit similar anti-inflammatory effects on LPS-induced RAW264.7 macrophages mainly through suppressing the MAPK and NF-κB signaling pathways, but with different intracellular mechanisms. These findings suggested that CBD may be considered a natural anti-inflammatory agent for protecting skin from immune disorders.
Authors: Hae Nim Lee; Seong Ah Shin; Gang Sik Choo; Hyeong Jin Kim; Young Seok Park; Byeong Soo Kim; Sang Ki Kim; Sung Dae Cho; Jeong Seok Nam; Chang Sun Choi; Jeong Hwan Che; Byung Kwon Park; Ji Youn Jung Journal: Int J Mol Med Date: 2017-11-29 Impact factor: 4.101
Authors: Mohammed I Khan; Anna A Sobocińska; Anna M Czarnecka; Magdalena Król; Bruno Botta; Cezary Szczylik Journal: Curr Pharm Des Date: 2016 Impact factor: 3.116