Preoperative neoadjuvant chemotherapy in patients with breast cancer evaluated using strain ultrasonic elastography.

BACKGROUND: The incidence of breast cancer in China is increasing while its mortality rate is decreasing. The annual breast cancer incidence in China is 39.2 million, accounting for two-thirds of the urban population. In China, breast cancer is the fifth most common malignant tumor overall and the most common in women, accounting for 17% of female malignant tumors. AIM: To investigate the accuracy of strain ultrasound elastography (SUE) on the evaluation of preoperative neoadjuvant chemotherapy (NAC) in breast cancer.
METHODS: Overall, 90 patients with breast cancer treated at our hospital between January 2018 and February 2019 were selected for this study. The patients received six cycles of NAC with docetaxel, epirubicin, and cyclophosphamide. Surgical treatment was also performed, and pathological reactivity was assessed. The patients were evaluated using conventional ultrasonography and SUE before biopsy. The differences between groups were analyzed to calculate the mean and standard deviation with significance measured using a t-test, while multivariate analysis was performed using logistic regression analysis.
RESULTS: Of the patients analyzed, 20 had a pathological complete remission (pCR) while 70 did not achieve pCR after NAC. The ratio of the elastic strain ratio (SR) and elastic score of 4-5 in patients with pCR were 5.5 ± 1.16 and 15.00%, respectively; these were significantly lower than those in patients without pCR (85%) and significantly higher than in patients without pCR (14%). SR and elastic score 4-5 were independent factors influencing NAC efficacy (OR=0.644, 1.426 and 1.366, respectively, P < 0.05). SR was positively correlated with elasticity score (rs = 0.411, P < 0.05). The area under the receiver operator characteristic curve of SR and SR combined with elastic score in predicting patients without pCR was 0.822 and 0.891, respectively (P < 0.05).
CONCLUSION: Strain ultrasonic elastography may be used to evaluate the effects of preoperative NAC in patients with breast cancer. ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.

Core Tip: The changes in a malignant tumor should be evaluated to determine the effects of preoperative neoadjuvant chemotherapy in breast cancer. However, conventional ultrasonography is not as sensitive as the current pathological reactivity detection method and may not meet clinical needs in determining the effect of neoadjuvant chemotherapy. A relatively novel method called strain ultrasound elastography was used in 90 patients with breast cancer in this study. It was found to be more effective and sensitive in the evaluation of the effects of neoadjuvant chemotherapy in patients with breast cancer.

INTRODUCTION

There is a high incidence of breast cancer in women. The use of adjuvant chemotherapy can effectively reduce mortality from breast cancer through treatment and prevention of recurrence[1]. Neoadjuvant chemotherapy (NAC) improves the efficacy of conventional treatments for breast cancer and helps prevent metastasis. However, there are individual differences in efficacy. Among these differences, pathological reactivity is an important factor affecting the efficacy of NAC. Therefore, it is necessary to predict the pathological reactivity of patients with breast cancer before starting NAC[2].

Strain ultrasound elastography (SUE) is in the stage of observation and research, and may be applied to the breast, thyroid, prostate, and other tissues of the body. The elastic coefficient between the lesion or tumor and the surrounding normal tissue produces different strain sizes and is displayed in different colors. This ultrasound technology has helped determine the size of malignant lesions through the detection of strain sizes[3]. This study aimed to investigate the accuracy of SUE in the evaluation of preoperative NAC and its related factors in patients with breast cancer.

MATERIALS AND METHODS

Study population

Ninety patients with breast cancer who were treated in our hospital between January 2018 and February 2019 were selected. They were 29–67 years old, with an average age of 49.80 ± 9.11 years. Of these, 78 patients were diagnosed with invasive ductal carcinoma and 12 with invasive lobular carcinoma. The inclusion criteria for the patients were as follows: female patients; informed consent provided by the patients and their families; SUE performed in our hospital; clinical stage II-III; completed six cycles of NAC consisting of docetaxel (75 mg/m2), epirubicin (75 mg/m2), and cyclophosphamide (500 mg/m2); underwent surgical treatment; and pathologically diagnosed with invasive carcinoma. The exclusion criteria for the patients were as follows: underwent radiotherapy, chemotherapy, and other anti-tumor treatments before the study period; had other systemic malignancies; or had other serious diseases such as immune system diseases, endocrine diseases, or liver and kidney dysfunction.

Assessment using strain ultrasound elastography

Conventional ultrasound and SUE were performed before tumor biopsy using our hospital’s HV800 ultrasound apparatus with a probe frequency of 8–12 Mhz. Conventional ultrasound examination of the breast was performed first to determine the tumor morphology, boundary, aspect ratio, surrounding tissue, echo, and blood flow changes. SUE was performed thereafter; the region of interest selected was more than twice the size of the tumor, the area with the tumor was exposed to slight vibration, compression frequency was 3–4 Hz, the depth of the probe was at the subcutaneous layer of the skin, adjacent to the muscle layer. The probe was placed perpendicular to the patient’s intact skin; the angle of the probe was adjusted in real time to maintain its perpendicular placement. Measurement of the elastic strain ratio (SR) of the axial adipose tissue to lesion was performed after stable image storage.

The images were assessed using a five-point scoring system[4], as follows: 1 point was assigned if the image was totally green, signifying a completely deformed tumor; 2 points, if the image was mainly green with visible blue, signifying that most of the tumor was deformed, but a small portion remained intact; 3 points, if the image of the area surrounding the tumor was green and deformed while the central area was blue and intact; 4 points, if the image of the tumor was blue overall and without any deformation; and 5 points, if the tumor and its surrounding tissue were blue, signifying no deformation. Hence, a score of 1 to 3 pertains to a low elasticity score, while a score of 4 to 5 pertains to a high elasticity score.

All patients received six cycles of NAC, consisting of docetaxel (75 mg/m2), epirubicin (75 mg/m2), and cyclophosphamide (500 mg/m2). They also underwent surgical treatment to determine their response to the chemotherapy through pathological examination. The patients who were assessed to have human epidermal growth factor receptor 2 positive tumors through fluorescence in situ hybridization received trastuzumab treatment. The doses of trastuzumab administered to the patients were 8 mg/kg body weight on the first cycle followed by 6 mg/kg body weight on the successive five cycles, with each cycle administered once every 3 wk.

Evaluation criteria

Pathological examination of the breast tissue was performed after surgical treatment (Figure 1). The Miller–Payne classification was used to determine pathological remission of malignancy through microscopic assessment. Based on this classification, grade 1 pertains to the unchanged number of invasive cancer cells; grade 2 to the less than 30% decrease in the amount of invasive cancer cells before chemotherapy; grade 3 to the 30%–90% decrease in the amount of invasive cancer cells compared with that before chemotherapy; grade 4 to more than 90% decrease in the amount of invasive cancer cells compared with that before chemotherapy, and with only scattered small cluster cells or single cancer cells retained; and grade 5 to pathological complete remission (pCR) where there are no invasive cancer cells in the original tumor bed. Grades 1–4 are defined as without pCR, and grade 5 is defined as with pCR.

Figure 1

Strain ultrasound elastography examination chart of a patient. A 45-year-old female patient with invasive ductal carcinoma, strain ultrasound elastography elasticity score of 4 points, strain ratio of 2.06, curative effect after neoadjuvant chemotherapy is pathological complete remission.

Statistical analysis

Data analysis was performed using SPSS 22.0 software. Nominal data were expressed as percentages. The differences between the groups, such as their SRs, were analyzed to calculate the mean ± SD and statistical significance analyzed using a t-test. Multivariate analysis was performed using logistic regression analysis; the prediction value of SR was analyzed using the receiver operator characteristic (ROC) curve. Statistical significance was set at P < 0.05.

RESULTS

Results of NAC chemotherapy

Pathological results of 90 patients after NAC chemotherapy: 14 cases of grade 1, 28 cases of grade 2, 17 cases of grade 3, 11 cases of grade 4, and 20 cases of grade 5. There were 20 pCR patients and 70 non-pCR patients.

Comparison of SR and elasticity scores between pCR and non-pCR patients

The ratio of SR and elasticity scores of 4 to 5 in pCR patients was significantly lower than that of non-pCR patients (P < 0.05, Table 1).

Table 1

Comparison of strain ratio and elasticity scores between pathological complete remission and non-pathological complete remission patients

Groups	Cases	SR	Elasticity score 4-5, n (%)
pCR	20	5.50 ± 1.16	3 (15.00)
Non-pCR	70	12.29 ± 6.60	54 (77.14)
t/χ2 value		-4.563	25.868
P value		0.000	0.000

pCR: Pathological complete remission; SR: Strain ratio.

Comparison of SR among patients with different elasticity scores

The clinical data were compared between patients with elasticity scores of 4 to 5 and 1 to 3 points, and the difference was not statistically significant (P > 0.05), as shown in Table 2. SR in patients with elastic score 4–5 was significantly higher than that in patients with elastic score 1–3 (P < 0.05). As shown in Table 3.

Table 2

Comparison of general clinical data of patients with different flexibility scores, n (%)

Groups	Elasticity score 4-5	Elasticity score 1-3	t value	P value
Cases	57	33
Age (yr)	50.06 ± 8.02	50.82 ± 9.11	-0.412	0.681
ER positive	35 (61.40)	18 (54.55)	0.406	0.524
PR positive	30 (52.63)	16 (48.48)	0.144	0.705
Ki-67 > 14%	32 (56.14)	19 (57.58)	0.018	0.895
HER2 positive	14 (24.56)	5 (15.15)	1.111	0.292
Lesion diameter (cm)	4.35 ± 1.13	4.30 ± 1.10	0.204	0.839

ER: Estrogen receptor; PR: Progesterone receptor; HER2: Human epidermal growth factor receptor 2.

Table 3

Comparison of SR among patients with different elasticity scores

Group	Cases	SR	t value	P value
Elasticity score 4-5	57	11.28 ± 1.43	4.271	0.000
Elasticity score 1-3	33	9.92 ± 1.50

SR: Strain ratio.

Comparison of general clinical data of pCR and non-pCR patients

The proportion of Ki-67 > 14% in pCR patients was significantly higher than that in non-pCR patients (P < 0.05). The age, lesion diameter, and estrogen receptor positivity were compared between pCR patients and non-pCR patients, and the difference was not statistically significant (P > 0.05, Table 4).

Table 4

Comparison of general clinical data of pathological complete remission and non-pathological complete remission patients, n (%)

General clinical data	pCR	Non-pCR	t /χ2 value	P value
Cases	20	70
Age (yr)	49.02 ± 8.19	51.02 ± 9.92	-0.824	0.412
Lesion diameter (cm)	4.42 ± 1.23	4.12 ± 1.20	0.981	0.329
ER positive	12 (60.00)	41 (58.57)	0.013	0.909
PR positive	11 (55.00)	35 (50.00)	0.156	0.693
Ki-67 > 14%	17 (85.00)	34 (48.57)	8.407	0.004
HER2 positive	7 (35.00)	12 (17.14)	2.003	0.157
Pathological type			0.386	0.534
Invasive ductal carcinoma	16 (80.00)	62 (88.57)
Invasive lobular carcinoma	4 (20.00)	8 (11.43)
Molecular subtypes			2.548	0.467
Triple negative	3 (15.00)	10 (14.29)
HER2	6 (30.00)	23 (32.86)
Luminal B	11 (55.00)	30 (42.86)
Luminal A	0 (0.00)	7 (10.00)

pCR: Pathological complete remission; ER: Estrogen receptor; PR: Progesterone receptor; HER2: Human epidermal growth factor receptor 2.

Correlation analysis

Spearman rank correlation analysis of the above indicators showed that SR was positively correlated with the elasticity score (rs = 0.411, P < 0.05), and there was no correlation between SR and elasticity score (Table 5).

Table 5

Correlation coefficient

Index	SR
	rs value	P value
Age	0.193	0.304
Lesion diameter	0.182	0.353
ER positive	0.082	0.405
PR positive	0.100	0.722
Ki-67 > 14%	0.092	0.565
HER2 positive	0.182	0.672
Elasticity score	0.411	0.000

SR: Strain ratio; ER: Estrogen receptor; PR: Progesterone receptor; HER2: Human epidermal growth factor receptor 2.

Multi-factor analysis

The clinical data of patients were used as independent variables, and the NAC efficacy was used as the dependent variable (pCR assignment 0, non-pCR assignment 1) for logistic regression analysis. The results showed that Ki-67 > 14%, SR, and elasticity scores 4–5 were independent influencing factors of NAC efficacy (OR = 0.644, 1.426, and 1.366, respectively, P < 0.05, Table 6).

Table 6

Logistic regression analysis

Factor	β	SE	Walds	P value	OR (95%CI)
Ki-67 > 14%	-0.440	0.132	11.111	0.000	0.644 (0.497-0.834)
SR	0.355	0.122	8.467	0.000	1.426 (1.123-1.811)
Elasticity score 4-5	0.312	0.2112	7.760	0.000	1.366 (1.097-1.702)

SR: Strain ratio.

SR, predicted value

The area under ROC curve of SR and SR combined with elasticity score in predicting non-pCR was 0.822 and 0.891, respectively (P < 0.05, Figure 2A and B, Table 7).

Figure 2

Receiver operator characteristic curve of strain ratio prediction and strain ratio joint elastic score prediction. A: Strain ratio (SR) prediction; B: SR joint elastic score prediction.

Table 7

Receiver operator characteristic curve parameters

Index	Area under curve	P value	Sensitivity	Specificity
SR	0.802	0.000	80.00%	70.00%
SR combined flexibility score	0.891	0.000	82.00%	83.00%

SR: Strain ratio.

DISCUSSION

NAC is an important supplement to the treatment of breast cancer and can significantly reduce the recurrence of malignant tumors in patients. It helps improve the prognosis of patients and the attainment of pCR. However, some previous studies[5-7] have found significant differences in the pathological response of individuals after NAC. The diagnosis of pathological remission is of great significance for individualized treatment and prognosis. However, there are differences in the sensitivity of the current NAC pathological reactivity detection methods, and simple conventional ultrasound cannot meet the clinical needs.

Previous literature[8-10] suggests that ultrasound elastography may have potential value in determining the pathological reactivity of malignant tumors after NAC, as patients with low elasticity scores were more likely to achieve complete tumor remission. Other studies[11,12] also suggested that the hardness of the tissue in breast cancer evaluated by ultrasound elastography was closely related to NAC reactivity. This study found that the SR and elasticity scores of 4–5 in patients with pCR were significantly lower than those in patients without pCR. The SR of patients with elasticity score of 4–5 was significantly higher than that of patients with elasticity score of 4–5. These results suggest that there is a low elasticity score in patients with breast cancer with complete remission; the proportion of SR lesions also decreased significantly. The SUE examination used the characteristics of uneven elastic coefficient distribution in the tissue; consequently, the strain distribution will also vary due to the unevenness of the elasticity in the tissue. The strain coefficient is small in an area with a large elastic coefficient; on the contrary, the strain coefficient is large in an area with a small elastic coefficient.

The Ki-67 antigen is a popular biological indicator in the study of various malignant tumors, especially breast cancer. Currently, Ki-67 antigen detection is generally used to describe cell proliferation in malignant tumors and to achieve targeted personalized treatment[13-15]. In this study, the comparison of clinical general data between patients with and without pCR showed that the proportion of Ki-67 was > 14% in patients with pCR and was significantly higher than that in patients without pCR. This suggests that the amount of Ki-67 expression in the tumor may be an important factor that affects the prognosis of NAC treatment in patients with breast cancer. The expression of Ki-67 in tumor proliferation covers all phases of the cycle except the G0 phase, hence the proportion of Ki-67 >14% in patients with pCR is higher. Related studies[16-18] also suggested that proliferating-cell nuclear antigen and Ki-67 could be used to detect the proliferation of breast cancer cells, and that they could be used as substitutes for each other. However, Ki-67 is more useful since it is expressed in all phases of the proliferative cycle of the malignant cells.

Multivariate analysis showed that Ki-67 > 14%, SR, and elasticity scores 4–5 independently influenced the efficacy of NAC. The clinical data of patients were the independent variables, and the NAC efficacy was the dependent variable. The SR values measured by SUE showed a high sensitivity and specificity in predicting pCR in patients with breast cancer. The expression of Ki-67 in low-differentiated adenocarcinoma tissues was significantly higher than that in medium and highly differentiated adenocarcinoma tissues; this indicates that the positive degree of Ki-67 staining was correlated with histological grade and was related to the occurrence and development of breast cancer. High Ki-67 expression signifies poor prognosis and is an important reference value for the diagnosis, treatment, and evaluation of breast cancer. SUE is an important supplement to the two-dimensional gray-scale ultrasound and can better evaluate the hardness of breast masses with good repeatability[19,20]. Unlike conventional two-dimensional gray-scale ultrasound, SUE can evaluate the hardness of lesions and indirectly reflect the composition of the tissue matrix. Therefore, the main potential benefit of SUE is a relatively accurate assessment of residual cancer after NAC. It is especially suitable for the assessment of cribriform, multinodular, and alternative atrophy patterns of residual cancer. Patients without pCR may have a non-uniform density of their breast mass that may be detected as a higher proportion of SR values and an elasticity score of 4–5 points. These explain the potential of the SR value and elasticity score of 4–5 points as predictors of the efficacy of NAC in patients with breast cancer.

CONCLUSION

Previous studies have mainly focused on the treatment and prognosis of patients with breast cancer after receiving NAC treatment. There are only a few studies and existing information on the prediction of pathological reactivity. This study analyzed and explored the pathological reactivity of breast cancer after NAC. It analyzed the possible mechanism of this change. This may potentially serve as a reference for the individualized treatment and prognosis of clinical NAC-assisted breast cancer treatment. In addition, this study also conducted an in-depth discussion on the diagnosis and efficacy analysis of breast cancer based on the relevant parameters of SUE technology that can provide a reference for clinical practice. However, this study also has a few shortcomings in that the sample size is relatively small and the findings will need to be confirmed by further large-sample studies.

ARTICLE HIGHLIGHTS

Research background

Breast cancer has the seventh highest mortality rate of all cancers in China and has the fifth highest cancer mortality in women, accounting for 8.2% of all female malignant tumors and seriously impacting women’s health and quality of life.

Research motivation

Early detection, diagnosis, and treatment are key to reducing mortality and improving cure rates.

Research objectives

Strain ultrasound elastography (SUE) is a new type of ultrasound technology that can determine relative tissue hardness by evaluating the desmoplastic changes in a tissue to differentiate between benign and malignant tumors.

Research methods

It is a way to diagnose tumors and diffuse diseases that cannot be detected by conventional ultrasound.

Research results

Of the patients analyzed, 20 had a pathological complete remission (pCR) while 70 did not achieve pCR after neoadjuvant chemotherapy (NAC).

Research conclusions

Strain ultrasonic elastography may be used to evaluate the effects of preoperative NAC in patients with breast cancer.

Research perspectives

SUE is a new type of ultrasound technology that can determine relative tissue hardness by evaluating the desmoplastic changes in a tissue to differentiate between benign and malignant tumors.