| Literature DB >> 36156789 |
Young Lo1, Shaolin Liang2,3, Waljit S Dhillo4, Anthony E G Cass5, Julian A Tanner6,7.
Abstract
Electrochemical aptamer-based (E-AB) sensors using conformational change-induced electron transfer kinetics are sensitive, reagent-less, and cost-effective tools for molecular sensing. Current advances in this technology can allow continuous drug pharmacokinetic monitoring in living animals (Dauphin-Ducharme et al., ACS Sens 4(10):2832-2837, 2019; Idili et al., Chem Sci 10(35):8164-8170, 2019), as well as automated analysis of hormone pulsatility (Liang et al., Nat Commun 10(1):852, 2019). In this chapter, we provide the methodology for an automated E-AB conformational change-based robotic sensing platform. By using an open-source programmable robotic system, this method can be adapted to a wide range of experimental scenarios.Entities:
Keywords: Aptamer; Aptasensor; Automated analysis; Biosensor; Electrochemical aptamer-based; Liquid-handling robot; Luteinizing hormone; Opentron; RAPTER; Squarewave voltammetry
Mesh：
Substances：
Year: 2023 PMID： 36156789 DOI： 10.1007/978-1-0716-2695-5_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745