Literature DB >> 36156787

Enzyme-Linked Oligonucleotide Assay (ELONA).

Miguel Moreno1, Ana García-Sacristán1, M Elena Martín2, Víctor M González3,4.   

Abstract

Aptamers are single-stranded oligonucleotides able to recognize a target with high affinity and specificity. Aptamers are used in different diagnostics applications, highlighting, among all, variations of the traditional enzyme-linked immunosorbent assay (ELISA). In this chapter, we show the procedures for the development of two types of indirect ELONA: a sandwich ELONA and a direct ELONA coupled to either real-time quantitative PCR as a direct and sensitive readout.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Affinity characterization; Aptamers; ELONA; ELONA qPCR; SELEX

Mesh：

Substances：

Year:  2023        PMID： 36156787     DOI： 10.1007/978-1-0716-2695-5_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Proceedings: ELISA, enzyme linked immunosorbent assay - a new technique for sero-diagnosis of trichinosis.

Authors:  I Ljungström; E Engvall; E J Ruitenberg
Journal:  Parasitology       Date:  1974-10       Impact factor: 3.234

2.  Comparison of Flow Cytometry and ELASA for Screening of Proper Candidate Aptamer in Cell-SELEX Pool.

Authors:  Maryam Sadat Nabavinia; Fahimeh Charbgoo; Mona Alibolandi; Fatemeh Mosaffa; Aida Gholoobi; Mohammad Ramezani; Khalil Abnous
Journal:  Appl Biochem Biotechnol       Date:  2017-07-19       Impact factor: 2.926

3.  Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance.

Authors:  Andrew L Chang; Maureen McKeague; Christina D Smolke
Journal:  Methods Enzymol       Date:  2014       Impact factor: 1.600

4.  Infectivity of hepatitis C virus correlates with the amount of envelope protein E2: development of a new aptamer-based assay system suitable for measuring the infectious titer of HCV.

Authors:  Ji Hoon Park; Min Hyeok Jee; Oh Sung Kwon; Sun Ju Keum; Sung Key Jang
Journal:  Virology       Date:  2013-02-26       Impact factor: 3.616

5.  Anti-Francisella tularensis DNA aptamers detect tularemia antigen from different subspecies by Aptamer-Linked Immobilized Sorbent Assay.

Authors:  Jeevalatha Vivekananda; Johnathan L Kiel
Journal:  Lab Invest       Date:  2006-06       Impact factor: 5.662

6.  Biophysical characterization of DNA aptamer interactions with vascular endothelial growth factor.

Authors:  Ajish S R Potty; Katerina Kourentzi; Han Fang; George W Jackson; Xing Zhang; Glen B Legge; Richard C Willson
Journal:  Biopolymers       Date:  2009-02       Impact factor: 2.505

7.  DNA aptamers selectively target Leishmania infantum H2A protein.

Authors:  M Elena Martín; Marta García-Hernández; Eva M García-Recio; Gerónimo F Gómez-Chacón; Marta Sánchez-López; Víctor M González
Journal:  PLoS One       Date:  2013-10-21       Impact factor: 3.240

8.  Efficient HIV-1 inhibition by a 16 nt-long RNA aptamer designed by combining in vitro selection and in silico optimisation strategies.

Authors:  Francisco J Sánchez-Luque; Michael Stich; Susanna Manrubia; Carlos Briones; Alfredo Berzal-Herranz
Journal:  Sci Rep       Date:  2014-09-01       Impact factor: 4.379

9.  Selection of a Novel Aptamer Against Vitronectin Using Capillary Electrophoresis and Next Generation Sequencing.

Authors:  Christopher H Stuart; Kathryn R Riley; Olcay Boyacioglu; Denise M Herpai; Waldemar Debinski; Shadi Qasem; Frank C Marini; Christa L Colyer; William H Gmeiner
Journal:  Mol Ther Nucleic Acids       Date:  2016-11-15       Impact factor: 10.183
  9 in total

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