Azza Gaber Antar Farag1, Azza Zagloul Labeeb2, Amany Nagy Abdalla Gerges3, Mustafa Elsayed Elshaib4. 1. Dermatology, Andrology and STDs Department, Faculty of Medicine, Menoufia University, Egypt. Electronic address: azzagaber92@yahoo.com. 2. Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Egypt. 3. Leprosy and Dermatology Clinic, Ministry of Health, Shebin Elkoom Teaching Hospital, Egypt. 4. Medical Student, Faculty of Medicine, Menoufia University, Egypt.
Abstract
BACKGROUND: Leprosy represents a long-term communicable disease resulting from Mycobacterium leprae infection. IL-17A is one of the pro-inflammatory cytokines that protects humans against many fungal and bacterial pathogens. OBJECTIVE: To investigate IL-17A (rs2275913) gene polymorphism and its circulating level in leprosy patients, and to correlate the detected results with different clinical aspects of leprosy in the investigated patients. METHODS: 60 patients with leprosy, and 29 age and sex-matched volunteers were investigated for IL-17A serum level and IL-17A single nucleotide polymorphism (SNP) by ELISA and RFLP-PCR respectively. RESULTS: IL-17A serum level was significantly higher in leprosy patients than in controls (p=0.034), and in TL than LL (p=0.017). IL-17A (rs2275913 A/G) G allele and GG genotype were associated significantly with LL (p=0.005and 0.001 respectively). IL-17A (rs2275913 A/G) AG genotype carriers demonstrated the highest IL-17A serum levels; however, its lowest levels were found in IL-17A (rs2275913 A/G) AA genotype carriers (p=0.005). Grade 2 disability (p=0.030) and positive slit skin smear (SSS) (p=0.005) were significantly associated with IL-17A (rs2275913 A/G) GG genotype. STUDY LIMITATIONS: The small number of studied subjects. CONCLUSIONS: IL -17A may have a pivotal role in leprosy pathogenesis. IL-17A (rs2275913) GG genotype plus G allele might be related to the development of LL in the Egyptian population.
BACKGROUND: Leprosy represents a long-term communicable disease resulting from Mycobacterium leprae infection. IL-17A is one of the pro-inflammatory cytokines that protects humans against many fungal and bacterial pathogens. OBJECTIVE: To investigate IL-17A (rs2275913) gene polymorphism and its circulating level in leprosy patients, and to correlate the detected results with different clinical aspects of leprosy in the investigated patients. METHODS: 60 patients with leprosy, and 29 age and sex-matched volunteers were investigated for IL-17A serum level and IL-17A single nucleotide polymorphism (SNP) by ELISA and RFLP-PCR respectively. RESULTS: IL-17A serum level was significantly higher in leprosy patients than in controls (p=0.034), and in TL than LL (p=0.017). IL-17A (rs2275913 A/G) G allele and GG genotype were associated significantly with LL (p=0.005and 0.001 respectively). IL-17A (rs2275913 A/G) AG genotype carriers demonstrated the highest IL-17A serum levels; however, its lowest levels were found in IL-17A (rs2275913 A/G) AA genotype carriers (p=0.005). Grade 2 disability (p=0.030) and positive slit skin smear (SSS) (p=0.005) were significantly associated with IL-17A (rs2275913 A/G) GG genotype. STUDY LIMITATIONS: The small number of studied subjects. CONCLUSIONS: IL -17A may have a pivotal role in leprosy pathogenesis. IL-17A (rs2275913) GG genotype plus G allele might be related to the development of LL in the Egyptian population.
Authors: Laurie E Harrington; Robin D Hatton; Paul R Mangan; Henrietta Turner; Theresa L Murphy; Kenneth M Murphy; Casey T Weaver Journal: Nat Immunol Date: 2005-10-02 Impact factor: 25.606
Authors: M B Santos; D T de Oliveira; R A Cazzaniga; C S Varjão; P L Dos Santos; M L B Santos; C B Correia; D R Faria; M do V Simon; J S Silva; W O Dutra; S G Reed; M S Duthie; R P de Almeida; A R de Jesus Journal: Scand J Immunol Date: 2017-07 Impact factor: 3.487
Authors: Xiaoqin Wu; Zhirong Zeng; Bin Chen; Jun Yu; Ling Xue; Yuantao Hao; Minhu Chen; Joseph J Y Sung; Pinjin Hu Journal: Int J Cancer Date: 2010-07-01 Impact factor: 7.396
Authors: Małgorzata Mazurek-Mochol; Małgorzata Kozak; Damian Malinowski; Krzysztof Safranow; Andrzej Pawlik Journal: Int J Environ Res Public Health Date: 2021-01-26 Impact factor: 3.390