Literature DB >> 36152203

DNA Methylation Analysis of Cnr1 Gene Promoter.

Claudio D'Addario1, Mariangela Pucci2.   

Abstract

DNA methylation pattern could be considered a biomarker to be exploited for the study and management of several human diseases. In this chapter, detailed protocols are provided for two experimental approaches used for quantitative methylation analysis of bisulfite converted DNA: methylation-specific PCR (MSP) and pyrosequencing.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  DNA extraction; DNA methylation; Methylation-specific PCR (MSP); Pyrosequencing; Type-1 cannabinoid receptor

Mesh：

Substances：

Year:  2023        PMID： 36152203     DOI： 10.1007/978-1-0716-2728-0_31

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

Authors:  K J Livak; T D Schmittgen
Journal:  Methods       Date:  2001-12       Impact factor: 3.608

2.  Bisulfite genomic sequencing: systematic investigation of critical experimental parameters.

Authors:  C Grunau; S J Clark; A Rosenthal
Journal:  Nucleic Acids Res       Date:  2001-07-01       Impact factor: 16.971

3.  Analysis of DNA Methylation by Pyrosequencing.

Authors:  Colin Delaney; Sanjay K Garg; Raymond Yung
Journal:  Methods Mol Biol       Date:  2015

Review 4.  Function and information content of DNA methylation.

Authors:  Dirk Schübeler
Journal:  Nature       Date:  2015-01-15       Impact factor: 49.962

5.  A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia.

Authors:  Claudio D'Addario; Vincenzo Micale; Martina Di Bartolomeo; Tibor Stark; Mariangela Pucci; Alexandra Sulcova; Mariacarlotta Palazzo; Zuzana Babinska; Laura Cremaschi; Filippo Drago; A Carlo Altamura; Mauro Maccarrone; Bernardo Dell'Osso
Journal:  Schizophr Res       Date:  2017-01-17       Impact factor: 4.939

6.  Extravirgin olive oil up-regulates CB₁ tumor suppressor gene in human colon cancer cells and in rat colon via epigenetic mechanisms.

Authors:  Andrea Di Francesco; Anastasia Falconi; Clara Di Germanio; Maria Vittoria Micioni Di Bonaventura; Antonio Costa; Stefano Caramuta; Michele Del Carlo; Dario Compagnone; Enrico Dainese; Carlo Cifani; Mauro Maccarrone; Claudio D'Addario
Journal:  J Nutr Biochem       Date:  2014-12-03       Impact factor: 6.048

7.  Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

Authors:  J G Herman; J R Graff; S Myöhänen; B D Nelkin; S B Baylin
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-03       Impact factor: 11.205

8.  Preclinical and Clinical Evidence for a Distinct Regulation of Mu Opioid and Type 1 Cannabinoid Receptor Genes Expression in Obesity.

Authors:  Mariangela Pucci; Maria Vittoria Micioni Di Bonaventura; Valeria Vezzoli; Elizabeta Zaplatic; Marcella Massimini; Stefania Mai; Alessandro Sartorio; Massimo Scacchi; Luca Persani; Mauro Maccarrone; Carlo Cifani; Claudio D'Addario
Journal:  Front Genet       Date:  2019-06-14       Impact factor: 4.599

Review 9.  DNA methylation biomarkers: cancer and beyond.

Authors:  Thomas Mikeska; Jeffrey M Craig
Journal:  Genes (Basel)       Date:  2014-09-16       Impact factor: 4.096
  9 in total

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