Literature DB >> 36152186

Assay of GTPγS Binding in Autoradiography.

Marina Gabaglio1, Pamela Prini1, Erica Zamberletti1, Tiziana Rubino2, Daniela Parolaro3.   

Abstract

Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant technique to study the function of G protein-coupled receptors (GPCRs) ex vivo. Here, we describe the protocol for such a method, suitable for investigating CB1 receptor functionality in tissue slices from rodent brains.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Keywords:  Autoradiography; Binding; Brain section; CB1 receptor; G-proteins; [35S]GTPγS

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Year:  2023        PMID： 36152186     DOI： 10.1007/978-1-0716-2728-0_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  1 in total

1.  Axonal transport of beta-adrenergic receptors. Antero- and retrogradely transported receptors differ in agonist affinity and nucleotide sensitivity.

Authors:  M A Zarbin; J M Palacios; J K Wamsley; M J Kuhar
Journal:  Mol Pharmacol       Date:  1983-09       Impact factor: 4.436
  1 in total

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