Literature DB >> 36152174

Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues.

Claudia Schwitter1, Beat Lutz2, Laura Bindila3.   

Abstract

Extraction and quantification of endocannabinoids from biological tissues is essential to unravel their changes under physiological and pathophysiological conditions. We describe here an analytical protocol for the extraction of endocannabinoids, anandamide (archidonoyl ethanolamide, AEA) and 2-arachidonoyl glycerol (2-AG), and endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tissues using liquid-liquid extraction method and simultaneous quantification by liquid chromatography multiple reaction monitoring (LC/MRM).
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Endocannabinoid-like lipids; Endocannabinoids; Lipid extraction; Lipidomics; Liquid-chromatography; Multiple reaction monitoring

Mesh：

Substances：

Year:  2023        PMID： 36152174     DOI： 10.1007/978-1-0716-2728-0_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Simultaneous lipidomic and transcriptomic profiling in mouse brain punches of acute epileptic seizure model compared to controls.

Authors:  Raissa Lerner; Julia M Post; Shane R Ellis; D R Naomi Vos; Ron M A Heeren; Beat Lutz; Laura Bindila
Journal:  J Lipid Res       Date:  2017-12-05       Impact factor: 5.922

2.  Therapeutic potential of inhibitors of endocannabinoid degradation for the treatment of stress-related hyperalgesia in an animal model of chronic pain.

Authors:  Ermelinda Lomazzo; Laura Bindila; Floor Remmers; Raissa Lerner; Claudia Schwitter; Ulrich Hoheisel; Beat Lutz
Journal:  Neuropsychopharmacology       Date:  2014-08-06       Impact factor: 7.853

3.  Adverse Social Experiences in Adolescent Rats Result in Enduring Effects on Social Competence, Pain Sensitivity and Endocannabinoid Signaling.

Authors:  Peggy Schneider; Laura Bindila; Christian Schmahl; Martin Bohus; Andreas Meyer-Lindenberg; Beat Lutz; Rainer Spanagel; Miriam Schneider
Journal:  Front Behav Neurosci       Date:  2016-10-20       Impact factor: 3.558

4.  A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner.

Authors:  María Teresa Ramírez-López; Mariam Vázquez; Ermelinda Lomazzo; Clementine Hofmann; Rosario Noemi Blanco; Francisco Alén; María Antón; Juan Decara; Rocío Arco; Laura Orio; Juan Suárez; Beat Lutz; Raquel Gómez de Heras; Laura Bindila; Fernando Rodríguez de Fonseca
Journal:  PLoS One       Date:  2017-03-27       Impact factor: 3.240

5.  Fear extinction learning and anandamide: an fMRI study in healthy humans.

Authors:  Jennifer Spohrs; Martin Ulrich; Georg Grön; Michael Prost; Paul Lukas Plener; Jörg Michael Fegert; Laura Bindila; Birgit Abler
Journal:  Transl Psychiatry       Date:  2021-03-15       Impact factor: 6.222
  5 in total

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