Literature DB >> 36152159

Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography-Mass Spectrometry.

Karolina Pietrowska1, Diana Anna Dmuchowska2, Adam Kretowski1, Michal Ciborowski3.   

Abstract

Aqueous humor (AH) is a transparent fluid that fills the anterior segment of the eye. The composition and level of metabolites in AH are important for understanding its physiology and changes caused by the occurrence of eye disease. A simple method for the preparation and analysis of AH samples was developed using the liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) technique. The analyses were performed using two types of chromatography: reversed-phase liquid chromatography-mass spectrometry (LC-RP-MS) and hydrophilic interaction liquid chromatography-mass spectrometry (LC-HILIC-MS), in the sample prepared by one protocol.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Aqueous humor; HILIC analysis; LC–MS; Reversed-phase analysis; Untargeted metabolomics

Mesh：

Year:  2023        PMID： 36152159     DOI： 10.1007/978-1-0716-2699-3_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

Review 1.  Analysis of pharmaceuticals and small molecules in aqueous humor.

Authors:  Karolina Pietrowska; Diana Anna Dmuchowska; Pawel Krasnicki; Zofia Mariak; Adam Kretowski; Michal Ciborowski
Journal:  J Pharm Biomed Anal       Date:  2018-06-25       Impact factor: 3.935

2.  An exploratory LC-MS-based metabolomics study reveals differences in aqueous humor composition between diabetic and non-diabetic patients with cataract.

Authors:  Karolina Pietrowska; Diana Anna Dmuchowska; Pawel Krasnicki; Aleksandra Bujalska; Paulina Samczuk; Ewa Parfieniuk; Tomasz Kowalczyk; Malgorzata Wojnar; Zofia Mariak; Adam Kretowski; Michal Ciborowski
Journal:  Electrophoresis       Date:  2018-01-19       Impact factor: 3.535
  2 in total

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