Victoria Gradaschi1,2, Florencia Payaslian1,2, Maria Eugenia Dieterle1,2, Liliana Rondón Salazar1,2, Estefanía Urdániz1,2, Matias Di Paola1,2, José Peña Cárcamo1,2, Fabio Zon1,2, Mariana Allievi1,2, Ezequiel Sosa3, Darío Fernandez Do Porto1,3, Matthew Dunne4, Pauline Goeller4, Jochen Klumpp4, Raúl Ricardo Raya5, Alejandro Reyes6,7, Mariana Piuri1,2. 1. Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. 2. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. 3. Instituto de Cálculo, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina. 4. Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland. 5. CERELA, CCT Conicet NOA Sur, SM Tucuman, Argentina. 6. Max Planck Tandem Group in Computational Biology, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia. 7. The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, Missouri, USA.
Abstract
Introduction: Only a few Lactobacillus casei phages have so far been characterized. As several L. casei strains are part of probiotic formulations, bacteriophage outbreaks targeting these strains can lead to critical losses within the dairy industry. Materials and Methods: A new L. casei phage was isolated from raw milk obtained from a milking yard from the province of Buenos Aires. The phage genome was sequenced, annotated, and analyzed. Morphology was determined by electron microscopy and the host range was established. Results: Lactobacillus phage vB_LcaM_Lbab1 is a member of the Herelleviridae family and features a host range including L. casei/Lactobacillus paracasei and Lactobacillus kefiri strains. We further analyzed the baseplate proteins in silico and found putative carbohydrate binding modules that are responsible for host recognition in other Lactobacillus phages. Conclusions: A new Lactobacillus phage was isolated and characterized. The focus was made on its host recognition mechanism, pointing toward the development of future strategies to avoid deleterious infections in the dairy industry. Copyright 2021, Mary Ann Liebert, Inc., publishers.
Introduction: Only a few Lactobacillus casei phages have so far been characterized. As several L. casei strains are part of probiotic formulations, bacteriophage outbreaks targeting these strains can lead to critical losses within the dairy industry. Materials and Methods: A new L. casei phage was isolated from raw milk obtained from a milking yard from the province of Buenos Aires. The phage genome was sequenced, annotated, and analyzed. Morphology was determined by electron microscopy and the host range was established. Results: Lactobacillus phage vB_LcaM_Lbab1 is a member of the Herelleviridae family and features a host range including L. casei/Lactobacillus paracasei and Lactobacillus kefiri strains. We further analyzed the baseplate proteins in silico and found putative carbohydrate binding modules that are responsible for host recognition in other Lactobacillus phages. Conclusions: A new Lactobacillus phage was isolated and characterized. The focus was made on its host recognition mechanism, pointing toward the development of future strategies to avoid deleterious infections in the dairy industry. Copyright 2021, Mary Ann Liebert, Inc., publishers.
Authors: François P Douillard; Angela Ribbera; Hanna M Järvinen; Ravi Kant; Taija E Pietilä; Cinzia Randazzo; Lars Paulin; Pia K Laine; Cinzia Caggia; Ingemar von Ossowski; Justus Reunanen; Reetta Satokari; Seppo Salminen; Airi Palva; Willem M de Vos Journal: Appl Environ Microbiol Date: 2013-01-11 Impact factor: 4.792
Authors: François P Douillard; Ravi Kant; Jarmo Ritari; Lars Paulin; Airi Palva; Willem M de Vos Journal: Microb Biotechnol Date: 2013-07-01 Impact factor: 5.813
Authors: Ambroise Desfosses; Hariprasad Venugopal; Tapan Joshi; Jan Felix; Matthew Jessop; Hyengseop Jeong; Jaekyung Hyun; J Bernard Heymann; Mark R H Hurst; Irina Gutsche; Alok K Mitra Journal: Nat Microbiol Date: 2019-08-05 Impact factor: 17.745