Ibrahim Besler1, Pavelas Sazinas2, Christian Harrison1, Lucy Gannon1, Tamsin Redgwell3, Slawomir Michniewski2, Steven P Hooton4, Jon L Hobman4, Andrew Millard1. 1. Department of Genetics and Genome Biology, University of Leicester, University Road, Leicester, United Kingdom. 2. Warwick Medical School, University of Warwick, Coventry, United Kingdom. 3. School of Life Sciences, University of Warwick, Coventry, United Kingdom. 4. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, United Kingdom.
Abstract
Background: Bacteriophages that infect Escherichia coli are relatively easily isolated, with >600 coliphage genomes sequenced to date. Despite this there is still much to be discovered about the diversity of coliphage genomes. Materials and Methods: Within this study, we isolated a coliphage from cattle slurry collected from a farm in rural England. Results: Transmission electron microscopy identified the phage as member of the Siphoviridae family. Phylogenetic analysis and comparative genomics further placed it within the subfamily Tunavirinae and forms part of a new genus. Conclusions: Characterization of the lytic properties of vB_Eco_SLUR29 reveals that it is rapidly able to lyse its host when infected at high multiplicity of infection, but not at low multiplicity of infection. Copyright 2020, Mary Ann Liebert, Inc., publishers.
Background: Bacteriophages that infect Escherichia coli are relatively easily isolated, with >600 coliphage genomes sequenced to date. Despite this there is still much to be discovered about the diversity of coliphage genomes. Materials and Methods: Within this study, we isolated a coliphage from cattle slurry collected from a farm in rural England. Results: Transmission electron microscopy identified the phage as member of the Siphoviridae family. Phylogenetic analysis and comparative genomics further placed it within the subfamily Tunavirinae and forms part of a new genus. Conclusions: Characterization of the lytic properties of vB_Eco_SLUR29 reveals that it is rapidly able to lyse its host when infected at high multiplicity of infection, but not at low multiplicity of infection. Copyright 2020, Mary Ann Liebert, Inc., publishers.
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