Aneuploidy determination in C-banded mouse metaphase II oocytes following cyclophosphamide treatment in vivo.

The ability of cyclophosphamide (CYC) to induce aneuploidy in metaphase II oocytes of mice was evaluated. Various dosages (50, 100, 150, 250 and 350 mg/kg body weight) of CYC were injected intraperitoneally prior to induced ovulation. Chromosomes were C-banded to allow a more accurate counting of chromosomes and chromatids, and analysis of structural aberrations. Structural aberrations were not observed. Although the incidence of aneuploid oocytes in the treated groups was greater than that in the control group, the differences were not statistically significant. This result was unlike those of other relevant reports and several plausible explanations for such a disparity are discussed.