Mitochondrial mutagenesis in yeast: mutagenic specificity of EMS and the effects of RAD9 and REV3 gene products.

EMS is capable of inducing point mutations in mitochondrial genomes of yeast. It induces efficiently the mitochondrial suppressor mutation of the mitochondrial ochre mutation oxi 1-V25. The base changes leading to the suppression effect have not been identified. AT----GC base substitutions in mitochondrial genomes are inefficiently induced by EMS. The RAD9 and REV3 gene products participate in EMS mutagenesis in nuclear, as well as mitochondrial genomes of yeast.