Incorporation of rat histocompatibility (AgB) antigens into liposomes, and their susceptibility to immune lysis.

The purification of detergent-solubilized rat histocompatibility antigens is described. The antigen may readily be incorporated into synthetic vesicles (liposomes) which appear to be unilamellar and between 0.1 microbeter and 0.2 micrometer in diameter. The addition of specific antibody leads to the agglutination and precipitation of the liposomes. An enzyme, horseradish peroxidase, may be incorporated into the trapped aqueous phase of the liposome, and its release by antibody and complement can be detected.