İbrahim Ethem1, Ceyhan Hacıoğlu1,2. 1. Düzce University, Faculty of Medicine, Department of Medical Biochemistry - Düzce, Turkey. 2. Düzce University, Faculty of Pharmacy, Department of Medical Biochemistry - Düzce, Turkey.
Abstract
OBJECTIVE: Heart attack is one of the most common causes of sudden death in adults. Therefore, early detection of heart attack and investigation of potential new biomarkers are of great importance. We investigated whether perilipin-5 is a potential biomarker by examining changes in perilipin-5 serum levels along with high-sensitivity cardiac troponin I during a heart attack. METHODS: The subjects were divided into two groups: (1) control group and (2) patients with heart attack, with 150 people in each group. High-sensitivity cardiac troponin I, perilipin-5, total oxidant status, malondialdehyde, reduced glutathione, and superoxide dismutase levels in serum samples were measured. In addition, perilipin-5 mRNA expressions and protein levels were analyzed. RESULTS: There was no overall statistical difference between the demographic characteristics of the groups. However, high-density lipoprotein, creatine kinase, Creatine kinase myocardial band, aspartate amino transferase, lactate dehydrogenase, and calcium levels were higher in the heart attack group compared to the control group. We found that the high-sensitivity cardiac troponin I and perilipin-5 levels increased in the patients with heart attack (p<0.0001) compared to control. Although there was an insignificant increase in malondialdehyde levels in the heart attack group (p>0.05), there was a 35.9% increase in total oxidant status levels and a 33.5 and 24.1% decrease in glutathione and superoxide dismutase levels, respectively (p<0.01), compared to control. Perilipin-5 mRNA and protein levels in heart attack patients increased by 48.2 and 23.6%, respectively, compared to the control group (p<0.01). CONCLUSION: Our results showed that perilipin-5 together with high-sensitivity cardiac troponin I could be a promising biomarker in heart attack.
OBJECTIVE: Heart attack is one of the most common causes of sudden death in adults. Therefore, early detection of heart attack and investigation of potential new biomarkers are of great importance. We investigated whether perilipin-5 is a potential biomarker by examining changes in perilipin-5 serum levels along with high-sensitivity cardiac troponin I during a heart attack. METHODS: The subjects were divided into two groups: (1) control group and (2) patients with heart attack, with 150 people in each group. High-sensitivity cardiac troponin I, perilipin-5, total oxidant status, malondialdehyde, reduced glutathione, and superoxide dismutase levels in serum samples were measured. In addition, perilipin-5 mRNA expressions and protein levels were analyzed. RESULTS: There was no overall statistical difference between the demographic characteristics of the groups. However, high-density lipoprotein, creatine kinase, Creatine kinase myocardial band, aspartate amino transferase, lactate dehydrogenase, and calcium levels were higher in the heart attack group compared to the control group. We found that the high-sensitivity cardiac troponin I and perilipin-5 levels increased in the patients with heart attack (p<0.0001) compared to control. Although there was an insignificant increase in malondialdehyde levels in the heart attack group (p>0.05), there was a 35.9% increase in total oxidant status levels and a 33.5 and 24.1% decrease in glutathione and superoxide dismutase levels, respectively (p<0.01), compared to control. Perilipin-5 mRNA and protein levels in heart attack patients increased by 48.2 and 23.6%, respectively, compared to the control group (p<0.01). CONCLUSION: Our results showed that perilipin-5 together with high-sensitivity cardiac troponin I could be a promising biomarker in heart attack.
Authors: Kristian Thygesen; Johannes Mair; Evangelos Giannitsis; Christian Mueller; Bertil Lindahl; Stefan Blankenberg; Kurt Huber; Mario Plebani; Luigi M Biasucci; Marco Tubaro; Paul Collinson; Per Venge; Yonathan Hasin; Marcello Galvani; Wolfgang Koenig; Christian Hamm; Joseph S Alpert; Hugo Katus; Allan S Jaffe Journal: Eur Heart J Date: 2012-06-21 Impact factor: 29.983
Authors: Tobias Reichlin; Raphael Twerenbold; Miriam Reiter; Stephan Steuer; Stefano Bassetti; Cathrin Balmelli; Katrin Winkler; Sabine Kurz; Claudia Stelzig; Michael Freese; Beatrice Drexler; Philip Haaf; Christa Zellweger; Stefan Osswald; Christian Mueller Journal: Am J Med Date: 2012-12 Impact factor: 4.965
Authors: Hong Wang; Urmila Sreenivasan; Da-Wei Gong; Kelly A O'Connell; Erinne R Dabkowski; Peter A Hecker; Nicoleta Ionica; Manige Konig; Anup Mahurkar; Yezhou Sun; William C Stanley; Carole Sztalryd Journal: J Lipid Res Date: 2013-01-23 Impact factor: 5.922
Authors: Christina Drevinge; Knut T Dalen; Maria Nastase Mannila; Margareta Scharin Täng; Marcus Ståhlman; Martina Klevstig; Annika Lundqvist; Ismena Mardani; Fred Haugen; Per Fogelstrand; Martin Adiels; Jorge Asin-Cayuela; Charlotte Ekestam; Jesper R Gådin; Yun K Lee; Hilde Nebb; Sara Svedlund; Bengt R Johansson; Lillemor Mattsson Hultén; Stefano Romeo; Björn Redfors; Elmir Omerovic; Max Levin; Li-Ming Gan; Per Eriksson; Linda Andersson; Ewa Ehrenborg; Alan R Kimmel; Jan Borén; Malin C Levin Journal: Int J Cardiol Date: 2016-06-16 Impact factor: 4.164
Authors: Nina M Pollak; Martina Schweiger; Doris Jaeger; Dagmar Kolb; Manju Kumari; Renate Schreiber; Stephanie Kolleritsch; Philipp Markolin; Gernot F Grabner; Christoph Heier; Kathrin A Zierler; Thomas Rülicke; Robert Zimmermann; Achim Lass; Rudolf Zechner; Guenter Haemmerle Journal: J Lipid Res Date: 2013-01-23 Impact factor: 6.676