Correlations of Computed Tomography Measurement of Distal Pulmonary Vascular Pruning with Airflow Limitation and Emphysema in COPD Patients.

Background: Pulmonary vascular alteration is an important feature of chronic obstructive pulmonary disease (COPD), which is characterized by distal pulmonary vascular pruning in angiography. We aimed to further investigate the clinical relevance of pulmonary vasculature in COPD patients using non-contrast computed tomography (CT).
Methods: Seventy-one control subjects and 216 COPD patients completed the questionnaires, spirometry, and computed tomography (CT) scans within 1 month and were included in the study. Small pulmonary vessels represented by percentage of cross-sectional area of pulmonary vessels smaller than 5 mm2 or 5-10 mm2 to the total lung fields (%CSA<5 or %CSA5-10, respectively) were measured using ImageJ software. Spearman correlation was used to investigate the relationship between %CSA<5 and airflow limitation. A receiver operating characteristic (ROC) curve was built to evaluate the value of %CSA<5 in discriminating COPD patients from healthy control subjects. Segmented regression was used to analyze the relationship between %CSA<5 and %LAA-950 (percentage of low-attenuation areas less than -950 HU).
Results: We found a significant correlation between %CSA<5 and forced expiratory volume in one second (FEV1) percentage of predicted value (%pred) (r = 0.564, P < 0.001). The area under the ROC curve for the value of %CSA<5 in distinguishing COPD was 0.816, with a cut-off value of 0.537 (Youden index J, 0.501; sensitivity, 78.24%; specificity, 71.83%). Since the relationship between %CSA<5 and %LAA-950 was not constant, performance of segmented regression was better than ordinary linear regression (adjusted R2, 0.474 vs 0.332, P < 0.001 and P < 0.001, respectively). As %CSA<5 decreased, %LAA-950 slightly increased until an inflection point (%CSA<5 = 0.524) was reached, after which the %LAA-950 increased apparently with a decrease in %CSA<5.
Conclusion: %CSA<5 was significantly correlated with both airflow limitation and emphysema, and we identified an inflection point for the relationship between %CSA<5 and %LAA-950.

Introduction

Chronic obstructive pulmonary disease (COPD) is a common disease characterized by airway or alveolar abnormalities. Recent studies have showed that pulmonary vascular alterations also involved in COPD.1 Previous studies demonstrated that the initial pulmonary vasculopathy in COPD patients was endothelial dysfunction, which led to a decrease in vascular tone and an increase in vascular resistance, and resulted in diminished ability to vasodilate and pulmonary hypertension (PH).1,2 In addition, hypoxia-induced vasoconstriction and vascular compression due to emphysema may aggravate pulmonary vascular pruning, referring to the narrowing, occlusion, and loss of peripheral pulmonary vessels.3 In most cases, clinicians do not have access to pulmonary vascular pathology because traditional vascular examination mainly includes invasive procedures, such as histopathology, right heart catheterization, and/or angiography.4–7 With the improvement in computed tomography (CT) technology and quantitative analysis, the pulmonary vessels may be evaluated noninvasively. The percentage of cross-sectional area (CSA) of small pulmonary vessels smaller than 5 mm2 to the total lung area (%CSA<5) is one of the most commonly used two-dimensional CT vascular parameters, which correlates with histological vascular CSA.8–10

Previous studies have found that %CSA<5 has a strong negative correlation with emphysema and a relatively weaker correlation with airflow limitation.10 Distal pulmonary vascular alterations have been demonstrated in smokers with normal lung function and individuals with mild emphysema.11–15 Together, these findings suggest that the relationships of peripheral pulmonary vascular alteration with airflow limitation and emphysema are inconsistent. We hypothesized that pulmonary vascular pruning may be significantly associated with emphysema and airflow limitation, but the association may change with disease progression. To test the hypothesis, we used %CSA<5 to quantify pulmonary vascular pruning and investigated the relationships of %CSA<5 with emphysema and airflow obstruction.

Materials and Methods

Participants

A cross-sectional observational study was prospectively conducted relying on the COPD Cohort of National Key R&D Program of China (NCT 03240315) in the First Affiliated Hospital of Guangzhou Medical University, China, between April 2017 and April 2021. This study included healthy control subjects (smoke or not, no known respiratory diseases or other systemic diseases, like cancer or autoimmune disease) and COPD patients, defined according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines16 (ratio of forced expiratory volume in 1 second [FEV1] to forced vital capacity [FVC] < 0.7 after using bronchodilator), and more than 40 years old. Patients were excluded if they had intrapulmonary diseases other than COPD, severe extrapulmonary diseases (for example, systemic inflammation or cancer), an exacerbation within the last 1 month, history of lung surgery or treatment with systemic glucocorticoids, or participation in other clinical trials. The demographic characteristics, smoking history (smoke status including never smoke, current smoke and ex-smoke which meant stop smoking for more than 6 months; Pack years), and symptomatic scores, including that of COPD assessment test (CAT) and modified British Medical Research Council dyspnea scale (mMRC), were collected from all participants using questionnaires. Spirometry was performed at the time of enrollment. Non-contrast CT scan was performed within 1 month after spirometry.

A total of 355 participants (potentially healthy controls and COPD patients) were enrolled, but 23 were excluded because of severe intrapulmonary diseases other than COPD or extrapulmonary diseases, such as severe bronchiectasis, stomach cancer, prostate cancer, and aortic dissection. In addition, 45 participants were excluded because chest CT scan and spirometry had not been performed within a month or the post-bronchodilator test had not been performed in those FEV1/FVC<0.7 (Figure 1). The study complied with the Declaration of Helsinki and was approved by the ethics committee of the hospital (approval no. 2017–22). All the participants provided informed consent before enrollment.

Figure 1

Flowchart of the study.

Pulmonary Function Tests

Spirometry was performed according to the American Thoracic Society (ATS)/European Respiratory Society (ERS) guidelines.17 Subjects whose FEV1/FVC<0.7 were required to test again after inhalation of bronchodilators. For these subjects, we recorded several indexes, including FEV1, FEV1/FVC, and FEV1%pred after using bronchodilator. COPD patients were classified according to the GOLD stage (GOLD 1: FEV1%pred > 80; GOLD 2: FEV1%pred 50–80; GOLD 3: FEV1%pred 30–50; GOLD 4: FEV1%pred < 30).

Chest CT

CT Acquisition

All participants underwent a non-contrast CT scan during full inspiration using a 64-slice multidetector CT scanner (Somatom Sensation 64; Siemens Healthineers AG, Erlangen, Germany). The scan was performed in the supine position. The parameters of the CT scan were as follows: 110 kV, 30 effective mAs, 64×0.6 mm collimation, a pitch of 1.3, rotation time 0.6 s, rotation field 400 mm, scan bed speed 146.4 mm/s, matrix 512*512. The CT data were reconstructed using a I31s kernel with 1 mm slice thickness and 1 mm increment. Then, a specific algorithm called SAFIRE was added for noise reduction.

Quantitative Measurement

Measurement of Small Pulmonary Vessels

To measure the CSA, three levels (1 cm above the upper margin of the aortic arch, 1 cm below the carina, and 1 cm below the right inferior pulmonary vein) were selected from the CT scan images. All selected slices were analyzed semi-automatically using an image processing software (ImageJ, version 1.52v). The threshold was set between −500 Hounsfield units (HU) and −1024 HU to segment the lung field. The image was converted to binary with a window level of −720 HU to display the pulmonary vessels in black. The circularity range was set at 0.9–1.0 and the size range was set at 0–5 mm2 or 5–10 mm2, respectively, to calculate the CSA of vessels. The CSA of the vessels and lung fields of the three selected slices were added (Figure 2). CSA<5 referred to the total CSA of vessels < 5 mm2 of the three slices; %CSA<5 referred to the percentage of the CSA<5 to the total lung area in the selected slices. CSA5–10 and %CSA5–10 were calculated in the same manner.9,10

Figure 2

(A) Segmented lung field by setting the threshold between −500 HU and −1024 HU; (B) Converted into binary image where pulmonary vessels were displayed in black with a window level of −720 HU; (C) Mask image after setting the circularity from 0.9 to 1.0 and the vascular size range from 0–5mm2.

Measurement of Large Pulmonary Vessels

The diameter of the main pulmonary artery (PA) at the level of the bifurcation or its tubular portion, and the mean of the two perpendicular diameters of the ascending aorta (AO) on the same image with a mediastinal window () was measured according to previous studies. The ratio of the PA to AO was used for analysis.18

To ensure consistency in measurements, CSA and PA:AO were independently measured by two respiratory physicians who were blinded to all of the clinical information. The average of the two measurements was used for analysis.

Measurements of Airway Wall and Emphysema

The CT data was imported into workstations of the VIDA software version 2.2 (Apollo; VIDA Diagnostics, Coralville, IA, USA) to automatically segment the lung and bronchial tree (). Then, the segmented bronchial trees were carefully reviewed and modified by an experienced respiratory clinician. To measure the structural airway changes, we obtained the average wall thickness (WT), wall area percentage (WA%), and thickness–diameter ratio (TDR) of the midpoint cross section of six bronchial segments, including the apical segment of right upper lobe (RB1), lateral segment of the right middle lobe (RB4), posterior segment of the right lower lobe (RB10), posterior segment of the left lower lobe (LB10), the apicoposterior segment (LB1+2), and the superior lingular segment (LB4) of the left upper lobe. These indexes were calculated automatically according to the formula as follows: WA% = (outer airway area − inner airway area)/outer airway area*100%; TDR = wall thickness/mean external diameter. The average value of the six segments was used for analysis. In addition, the percentage of low-attenuation areas (ie, < −950 HU) (%LAA-950) was calculated to evaluate the degree of emphysema.19

Statistical Analysis

SPSS software version 25.0 (IBM Corp., Armonk, NY, USA) was used to analyze the characteristics of participants. Continuous variables are presented as mean with standard deviation or median with interquartile ranges, based on the results of the normality test. Categorical variables are presented as number of subjects. Independent t-test and Mann–Whitney U-test were used to compare continuous variables, as appropriate. Chi-square test was used to compare the categorical variables between healthy controls and COPD patients. Linear regression was used for the trend test of all variables across the GOLD stages. Intraclass correlation coefficients (ICC) were calculated to identify the intra-observer and inter-observer agreement for %CSA<5, %CSA5-10, PA:AO. Spearman correlation was used to analyze the relationship between %CSA<5 and FEV1%pred. The classification performance of %CSA<5 for discriminating COPD patients was evaluated using receiver operating characteristic (ROC) curves. A segmented package in the R (R version 3.5.3) (a statistical method offering piecewise linear regression of a given dataset and further providing the inflection point where the slope of the regression line suddenly changes) was used to further explore the relationship between %CSA<5 and emphysema and to determine the inflection point of the correlation. Finally, independent t-test, Mann–Whitney U-test, and chi-square test were used to compare the characteristics between subjects above and below the inflection point.

Results

Study Population

Among a total of 355 individuals screened for study inclusion, 287 (37 never smoking control subjects, 34 smoking control subjects and 216 COPD patients) were included in the study. The ICC value for intra-observer agreement for %CSA<5, %CSA5–10, PA:AO were 0.973 (0.933–0.989), 0.768 (0.497–0.901) and 0.710 (0.443–0.856), respectively. And ICC value for inter-observer agreement were 0.954 (0.942–0.963), 0.812 (0.768–0.848) and 0.866 (0.834–0.892), respectively. The clinical characteristics of eligible subjects, including healthy control subjects smoke or not, and COPD patients classified according to the GOLD stage, are presented in Table 1. The CT vascular parameters (including %CSA<5, %CSA5–10, and PA:AO), as well as emphysema index (%LAA-950) and airway wall thickness (WT), were not significantly different between never smoking and smoking control subjects, but were significant different between COPD patients and healthy control subjects. Both %CSA<5 and %CSA5–10 generally decreased, while emphysema severity increased with worsened airflow limitation.

Table 1

Characteristics of Study Participants

Characteristic	Never-Smoking Control Subjects	Smoking Control Subjects	GOLD 1	GOLD2	GOLD3	GOLD4	P1	P2	P3
Subject n	37	34	30	91	75	20
Age (years)	56.38±9.70	58.41±7.11	67.40±7.04	67.54±9.02	65.51±7.08	63.50±7.72	0.315	0.029	<0.001
Sex							<0.001	0.078	<0.001
Male	14	32	25	85	72	19
Female	23	2	5	6	3	1
BMI (kg/m2)	24.27(22.67–26.59)	25.63(22.73–27.40)	21.13 (20.40–24.42)	24.11 (22.26–26.03)	21.13 (18.39–23.37)	21.06 (20.25–22.70)	0.44	<0.001	<0.001
mMRC dyspnea score							0.772	<0.001	<0.001
0	14	18	6	13	3	0
1	8	8	20	44	22	0
2	3	2	4	27	32	7
3	0	0	0	3	15	12
4	0	0	0	2	1	1
CAT score	2 (1–5.75)	4 (2–8)	7(5–9)	7(5–10)	10(7–14)	13.5(10–20.25)	0.163	<0.001	<0.001
Smoking status							<0.001	0.171	<0.001
Never-smoker	37	-	9	13	4	1
Ex-smoker	-	15	9	49	42	14
Current smoker	-	19	12	29	29	5
Pack-years	-	30(14.38–40)	23 (0–39)	26.25 (9.73–40)	40 (22.5–60)	40 (31.25–50)	<0.001	<0.001	<0.001
%CSA<5(%)	0.60 (0.55–0.78)	0.61 (0.46–0.71)	0.50 (0.42–0.58)	0.47 (0.41–0.55)	0.38 (0.32–0.45)	0.35 (0.30–0.41)	0.105	<0.001	<0.001
%CSA5–10(%)	0.31 (0.24–0.38)	0.29 (0.24–0.34)	0.23 (0.21–0.28)	0.25 (0.21–0.29)	0.20 (0.15–0.24)	0.18 (0.16–0.20)	0.369	<0.001	<0.001
PA:AO	0.86 (0.81–0.97)	0.82 (0.75–0.94)	0.84 (0.73–0.91)	0.81 (0.73–0.88)	0.83 (0.75–0.88)	0.83 (0.80–0.91)	0.173	0.484	0.007
%LAA-950	0.43 (0.26–1.15)	0.68 (0.31–1.27)	2.06 (0.66–4.72)	4.46 (1.52–11.41)	18.97 (9.07–27.16)	25.83 (18.64–38.36)	0.239	<0.001	<0.001
WT (mm)	1.57 (1.51–1.65)	1.66 (1.58–1.69)	1.59 (1.50–1.69)	1.61 (1.48–1.69)	1.54 (1.45–1.60)	1.55 (1.45–1.62)	0.013	0.088	0.005
WA (%)	0.64 (0.63–0.68)	0.65 (0.61–0.67)	0.65 (0.62–0.66)	0.66 (0.61–0.68)	0.66 (0.64–0.67)	0.66 (0.63–0.67)	0.555	0.663	0.233
TDR	0.20 (0.19–0.21)	0.20 (0.19–0.20)	0.20 (0.18–0.20)	0.20 (0.18–0.21)	0.20 (0.19–0.21)	0.20 (0.19–0.21)	0.491	0.641	0.403

Notes: The continuous variable are presented as mean±SD or median with IQR as appropriate; categorical variables are presented as number of subjects; P1-value: comparison between never-smoking control subjects and smoking control subjects. P2-value: trend test across GOLD stage. P3-value: comparison between healthy control subjects and COPD patients.

Abbreviations: BMI, body mass index; mMRC dyspnea score, modified British Medical Research Council dyspnea score; CAT score, the score of a questionnaire call COPD assessment test; %CSA<5, the percentage of total cross-sectional area of pulmonary vessels <5 mm2 to the total lung area of the selected slices; %CSA5–10, the percentage of total cross-sectional area of pulmonary vessels among 5–10 mm2 to the total lung area of the selected slices; PA:AO ratio, the ratio of diameter of the main pulmonary artery (PA) to the diameter of ascending aorta (AO) at the level of the bifurcation; %LAA-950, Percentage of low-attenuation areas less than −950 Hounsfield units to the total lung volume; WT, mean wall area of this airway; WA%, airway wall percentage; TDR, thickness–diameter ratio;

Correlation Between %CSA<5 and Airflow Limitation

The %CSA<5 was significantly higher in healthy individuals than COPD patients, and gradually decreased with increasing GOLD stage in COPD patients, indicating that %CSA<5 was associated with airflow limitation and may be used to identify COPD patients. As presented in Figure 3, %CSA<5 was significantly correlated with FEV1%pred (r = 0.564, P < 0.001). Then, we constructed an ROC curve to investigate the value of %CSA<5 in discriminating COPD patients from individuals without airflow limitation. As a result, we obtained the area under the curve (AUC) of 0.816, with a cut-off value of 0.537 (Youden index J,0.501; sensitivity, 78.24%; specificity, 71.83%) (Figure 4).

Figure 3

Relationship between %CSA<5 and FEV1%pred (r=0.564, P<0.001).

Figure 4

ROC curve of %CSA<5 for the discrimination of COPD. (AUC, 0.816; cut-off value, 0.537; Youden index J, 0.501; sensitivity, 78.24%; specificity, 71.83%).

Correlation Between %CSA<5 and Emphysema

To explore the relationship between %CSA<5 and emphysema, we established an ordinary linear regression model and a segmented regression model. As shown in Figure 5, the predictive ability of segmented regression (adjusted R2, 0.474; P < 0.001) was superior to that of ordinary linear regression (adjusted R2, 0.332, P < 0.001). According to the segmented regression model, before %CSA<5 reached the inflection point (0.524), the regression line was nearly flat (β1 = −3.104, P < 0.001), while the slope of the regression line suddenly increased when the %CSA<5 went below the inflection point (β2 = −96.615, P < 0.001). Compared with individuals with %CSA<5 higher than 0.524, subjects with %CSA<5 less than 0.524 had elder ages, lower body mass index, lower PA:AO and exhibited more severe symptoms, emphysema, and airflow limitation (Table 2).

Figure 5

Association between %CSA<5 and %LAA-950. The fitting effect of segmented regression (adjust R2, 0.474; P <0.001) was much better than ordinary linear regression (adjust R2, 0.332, P<0.001). The slope of the ordinary linear regression line was β0 = −41.308. In the segmented regression, before %CSA<5 decrease to inflection point (0.524), the regression line was nearly flat (β1 = −3.104, P < 0.001), while the slope of the regression line suddenly flared up when the %CSA<5 went down below the inflection point (β2 = −96.615, P < 0.001).

Table 2

Comparison of Characteristics of Participants Above and Below the Inflection Point

%CSA<5	≥0.524	<0.524	P
Subjects n	104	183
Age (years)	62.60±1.03	65.86±0.61	<0.001
Sex			<0.001
Male	75	172
Female	29	11
BMI (kg/m2)	24.18±0.35	22.29±0.25	<0.001
mMRC dyspnea score			<0.001
0	31	23
1	37	65
2	15	60
3	3	27
4	1	3
CAT score	6 (2.5–10)	8 (6–11)	<0.001
Smoking status			<0.001
Never-smoker	40	24
Ex-smoker	32	97
Current smoker	32	62
Pack-years	22.50 (0–40)	30.40 (12.50–49.15)	<0.001
FEV1%pred	83.30 (66.60–100.75)	52.60 (36.10–75.40)	<0.001
PA:AO	0.87 (0.78–0.94)	0.81 (0.73–0.88)	<0.001
%LAA-950	0.68 (0.36–2.23)	12.00 (3.54–23.94)	<0.001
WT (mm)	1.61 (1.50–1.69)	1.58 (1.48–1.64)	0.153
WA (%)	0.65 (0.61–0.67)	0.65 (0.63–0.67)	0.464
TDR	0.20 (0.18–0.21)	0.20 (0.19–0.21)	0.908

Notes: The continuous variable are presented as mean±SD or median with IQR as appropriate; categorical variables are presented as number of subjects; P-value: comparison between subjects upper and below the inflection point.

Abbreviations: BMI, body mass index; mMRC dyspnea score, modified British Medical Research Council dyspnea score; CAT score, the score of a questionnaire call COPD assessment test; %CSA<5, the percentage of total cross-sectional area of pulmonary vessels <5 mm2 to the total lung area of the selected slices; %CSA5–10, the percentage of total cross-sectional area of pulmonary vessels among 5–10 mm2 to the total lung area of the selected slices; PA:AO ratio, the ratio of diameter of the main pulmonary artery (PA) to the diameter of ascending aorta (AO) at the level of the bifurcation; %LAA-950, Percentage of low-attenuation areas less than −950 Hounsfield units to the total lung volume; WT, mean wall area of this airway; WA%, airway wall percentage; TDR, thickness–diameter ratio.

Discussion

In current study, our goal was to investigate the clinical relevance of distal pulmonary vascular pruning in COPD, which was mainly evaluated by %CSA<5. We found that peripheral pulmonary vascular pruning significantly correlated with airflow limitation, and %CSA<5 had a potential to discriminate patients of COPD from individuals without airflow limitation. Another interesting finding was that %CSA<5 was not always strongly negatively associated with emphysema. When %CSA<5 was greater than the inflection point (0.524), correlation between %CSA<5 and %LAA-950 was weak but statistically significant. When %CSA<5 decreased below the inflection point, the correlation sharply increased.

Pulmonary vascular alteration is a significant component of the pathophysiological mechanism of COPD. Histological studies demonstrated that smoking causes peripheral pulmonary vascular alteration, mainly including intimal thickening, smooth muscle cell hyperplasia, and elastin deposition.1,2 Furthermore, pulmonary angiography demonstrated peripheral pulmonary vascular pruning in smokers.3,20 Such vascular changes are also present in COPD patients given that smoking is one of the most significant risk factors of COPD.1,2,7 In our study, there was no significant difference in %CSA<5 between never-smoking and smoking control subjects, which we attributed to insufficient sample size. However, %CSA<5 in COPD patients was significantly lower than that in healthy individuals, which may further decrease with worsening airflow obstruction, indicating that %CSA<5 may be helpful for the discrimination and severity stratification of COPD. This hypothesis was proved to be reasonable since %CSA<5 had a significant correlation with FEV1%pred in our study. The ROC curve was built to investigate the value of %CSA<5 in discriminating COPD and obtained an AUC of 0.816 and a cut-off value of 0.537, implying that %CSA<5 has a potential for COPD identification; however, this must be tested in further studies with larger populations.

Airflow obstruction was affected by both small airway disease and emphysema. Distal pulmonary vascular pruning was closely associated with emphysema.2,3,21,22 Analysis of the COPDGene, a cohort with the aims of exploring the genetic risk factors as well as imaging features of COPD, demonstrated a strong correlation between pulmonary vessel pruning and emphysema,10 but there was no such association in the Framingham cohort, a general adult cohort with fewer participants with emphysema and airflow limitation.23 Our findings explain this inconsistency. In our study, %CSA<5 did not always show a strong negative correlation with %LAA-950. The negative correlation was evident after %CSA<5 declined to an inflection point (0.524). Pulmonary vasculopathy in COPD patients occurs because of smoking-induced endothelial dysfunction, which is also a notable cause of emphysema. This may be the reason for the slow rise in %LAA-950 as %CSA<5 decreased in patients with mild vascular pruning. As the disease progressed, development of peripheral vascular pruning and emphysema would result in ventilation-perfusion ratio (V/Q) mismatch, impaired gas exchange, and finally aggravation of hypoxia. Hypoxia not only causes pulmonary vasoconstriction but also causes an increase in the respiratory rate, which may accelerate the progression of emphysema and worsen the V/Q ratio mismatch.1,2,7 These changes eventually form a vicious circle that amplifies the influence of pulmonary vascular pruning on emphysema and airflow obstruction. This may be the reason for the sudden change in the relationship between %CSA<5 and %LAA-950 with disease progression. To the best of our knowledge, this is the first study to examine the relationship between peripheral pulmonary vascular pruning and emphysema using piecewise regression. In this study, for the first time, we demonstrated an inflection point of %CSA<5, which marked the beginning of its strong correlation with emphysema.

On the basis of these results, pulmonary vascular alteration was significantly correlated with airflow limitation and emphysema. The inflection point implied that when %CSA<5 decreased to a certain level, the destruction of lung parenchyma and impairment of lung function were accelerated. At that time, the traditional treatment strategies were insufficient and had to be coupled with appropriate pulmonary vascular therapy. In clinical work, the symptoms of COPD patients like degree of dyspnea often do not match the degree of airflow limitation and many COPD patients do not achieve symptom relief with traditional treatments.1,24–26 It is necessary to consider whether such patients are affected by pulmonary vascular alterations. In addition, Coster et al have demonstrated that %CSA<5 was one of the best predictors of mean pulmonary arterial pressure in COPD patients with and without severe pulmonary hypertension.27 Therefore, CT vascular measurement was expected to be a useful screening tool for PH in COPD patients and a noninvasive tool for the assessment of disease condition and therapeutic efficacy. All in all, CT vascular measurement showed considerable value for clinical application.

This study had some limitations that need to be addressed. First, CSA of pulmonary vessels was measured using a semi-automatic and two-dimensional method, which was time-consuming and may be affected by inter-measurer variation.9,10 To reduce this variation, we requested two independent physicians to record the measurements and calculated the average of the two measurements for analysis. Second, this was a cross-sectional observational study; therefore, we could not investigate the correlations of pulmonary vascularity with COPD exacerbation, progression, and prognosis.

Conclusion

CT measurement of pulmonary vascular pruning was associated with airflow obstruction, and %CSA<5 showed considerable potential to discriminate COPD patients from individuals without persistent airflow limitation. The relationship between pulmonary vascular pruning and emphysema was not invariable; rather, the relationship abruptly increased when %CSA<5 reduced below the inflection point.