| Literature DB >> 36127730 |
Abstract
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Year: 2022 PMID: 36127730 PMCID: PMC9487105 DOI: 10.1186/s13048-022-01038-5
Source DB: PubMed Journal: J Ovarian Res ISSN: 1757-2215 Impact factor: 5.506
Fig. 1Expression pattern and functional role of PAXIP1-AS1 in OC cells. a RT-qPCR data of PAXIP1-AS1 expression in HOSEpiC cell line and OC cell lines. b Knockdown of PAXIP1-AS1 in SKOV3 and OVCAR3 cells validated by RT-qPCR. c-d Proliferation of SKOV3 and OVCAR3 cells upon PAXIP1-AS1 silencing was evaluated via colony formation assay and EdU assay. e Apoptosis of SKOV3 and OVCAR3 cells after PAXIP1-AS1 silencing was assessed through flow cytometry analysis. f Protein levels of Bax, Bcl-2, caspase-3 and caspase-9 under sh-PAXIP1-AS1 transfection were detected by western blot. g Migration of SKOV3 and OVCAR3 cells transfected with sh-PAXIP1-AS1 was confirmed by Transwell assay. h MMP2, MMP9, E-cadherin and N-cadherin protein levels were testified with western blot upon PAXIP1-AS1 knockdown. *p < 0.05
Fig. 5PCBP2 was a target of PAXIP1-AS1 in regulating OC cellular process. a Expression of PCBP2 in cells transfected with pcDNA3.1/PCBP2. b-c Cell proliferation with indicated transfection was tested by colony formation and EdU assays. d-e Apoptotic rate and levels of apoptosis-relevant proteins were respectively determined by flow cytometry analysis and western blot. f Cell migration in each group was measured through Transwell assay. g Levels of migration-related proteins and EMT-associated proteins in cells transfected with appointed plasmids were evaluated using western blot. *p < 0.05