| Literature DB >> 36127582 |
Wayne B Anderson1, Putuma P Gqamana1, Y Victoria Zhang2.
Abstract
Cortisol is one of the most important glucocorticoids involved in the regulation of human metabolism and physiological stress. Monitoring of levels of cortisol is of immense clinical benefit. In particular, salivary cortisol levels have been shown to correlate well with diurnal changes in cortisol levels in serum and have been used widely for monitoring of cortisol levels for diagnosis and prognosis purposes. We present a sensitive, fast, and robust quantitative liquid chromatography and tandem mass spectrometry (LC-MS/MS) assay for salivary cortisol in negative mode. This assay employs protein precipitation followed by reversed-phase liquid chromatographic separation, negative-mode electrospray ionization (ESI), and MS/MS detection. This assay has a total run time of 5.8 minutes and a limit of quantification of 0.5 ng/mL with a linear range up to 100 ng/mL. No carryover was observed at 10 μg/mL. This assay also incorporates the routine monitoring of prednisolone, a potential interferent to salivary cortisol.Entities:
Keywords: Cushing’s syndrome; Interferents; LC-MS/MS; Prednisolone; Quality control; Salivary cortisol
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Year: 2022 PMID： 36127582 DOI： 10.1007/978-1-0716-2565-1_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745