Literature DB >> 36125764

Methods to Analyze Motility in Eury- and Crenarchaea.

Megha Patro1,2, Marleen van Wolferen1, Xing Ye1, Sonja-Verena Albers3, Tessa E F Quax4,5.   

Abstract

Many archaea display swimming motility in liquid medium, which is empowered by the archaellum. Directional movement requires a functional archaellum and a sensing system, such as the chemotaxis system that is used by Euryarchaea. Two well-studied models are the euryarchaeon Haloferax volcanii and the crenarchaeon Sulfolobus acidocaldarius. In this chapter we describe two methods to analyze their swimming behavior and directional movement: (a) time-lapse microscopy under native temperatures and (b) spotting on semi-solid agar or gelrite plates. Whereas the first method allows for deep analysis of swimming behavior, the second method is suited for high throughput comparison of multiple strains.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Archaea; Archaellum; Haloferax; Motility; Semi-solid agar plates; Sulfolobus; Thermomicroscopy; Time-lapse microscopy

Mesh：

Substances：

Year:  2022        PMID： 36125764     DOI： 10.1007/978-1-0716-2445-6_25

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  23 in total

1.  Car: a cytoplasmic sensor responsible for arginine chemotaxis in the archaeon Halobacterium salinarum.

Authors:  K F Storch; J Rudolph; D Oesterhelt
Journal:  EMBO J       Date:  1999-03-01       Impact factor: 11.598

2.  Molecular analysis of the crenarchaeal flagellum.

Authors:  Kerstin Lassak; Tomasz Neiner; Abhrajyoti Ghosh; Andreas Klingl; Reinhard Wirth; Sonja-Verena Albers
Journal:  Mol Microbiol       Date:  2011-11-20       Impact factor: 3.501

3.  The archaellum: an old motility structure with a new name.

Authors:  Ken F Jarrell; Sonja-Verena Albers
Journal:  Trends Microbiol       Date:  2012-05-19       Impact factor: 17.079

Review 4.  The Archaellum: An Update on the Unique Archaeal Motility Structure.

Authors:  Sonja-Verena Albers; Ken F Jarrell
Journal:  Trends Microbiol       Date:  2018-02-13       Impact factor: 17.079

5.  A novel mode of sensory transduction in archaea: binding protein-mediated chemotaxis towards osmoprotectants and amino acids.

Authors:  Maia V Kokoeva; Kai-Florian Storch; Christian Klein; Dieter Oesterhelt
Journal:  EMBO J       Date:  2002-05-15       Impact factor: 11.598

6.  Direct observation of rotation and steps of the archaellum in the swimming halophilic archaeon Halobacterium salinarum.

Authors:  Yoshiaki Kinosita; Nariya Uchida; Daisuke Nakane; Takayuki Nishizaka
Journal:  Nat Microbiol       Date:  2016-08-26       Impact factor: 17.745

7.  Systematic deletion analyses of the fla genes in the flagella operon identify several genes essential for proper assembly and function of flagella in the archaeon, Methanococcus maripaludis.

Authors:  Bonnie Chaban; Sandy Y M Ng; Masaomi Kanbe; Ilana Saltzman; Graeme Nimmo; Shin-Ichi Aizawa; Ken F Jarrell
Journal:  Mol Microbiol       Date:  2007-09-20       Impact factor: 3.501

8.  Deletion analysis of the che operon in the archaeon Halobacterium salinarium.

Authors:  J Rudolph; D Oesterhelt
Journal:  J Mol Biol       Date:  1996-05-17       Impact factor: 5.469

9.  Structure and function of the archaeal response regulator CheY.

Authors:  Tessa E F Quax; Florian Altegoer; Fernando Rossi; Zhengqun Li; Marta Rodriguez-Franco; Florian Kraus; Gert Bange; Sonja-Verena Albers
Journal:  Proc Natl Acad Sci U S A       Date:  2018-01-22       Impact factor: 11.205

10.  Taxis in archaea.

Authors:  Tessa E F Quax; Sonja-Verena Albers; Friedhelm Pfeiffer
Journal:  Emerg Top Life Sci       Date:  2018-12-14
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