MAIN CONCLUSION: The overexpression of the GmGlb1-1 gene reduces plant susceptibility to Meloidogyne incognita. Non-symbiotic globin class #1 (Glb1) genes are expressed in different plant organs, have a high affinity for oxygen, and are related to nitric oxide (NO) turnover. Previous studies showed that soybean Glb1 genes are upregulated in soybean plants under flooding conditions. Herein, the GmGlb1-1 gene was identified in soybean as being upregulated in the nematode-resistant genotype PI595099 compared to the nematode-susceptible cultivar BRS133 during plant parasitism by Meloidogyne incognita. The Arabidopsis thaliana and Nicotiana tabacum transgenic lines overexpressing the GmGlb1-1 gene showed reduced susceptibility to M. incognita. Consistently, gall morphology data indicated that pJ2 nematodes that infected the transgenic lines showed developmental alterations and delayed parasitism progress. Although no significant changes in biomass and seed yield were detected, the transgenic lines showed an elongated, etiolation-like growth under well-irrigation, and also developed more axillary roots under flooding conditions. In addition, transgenic lines showed upregulation of some important genes involved in plant defense response to oxidative stress. In agreement, higher hydrogen peroxide accumulation and reduced activity of reactive oxygen species (ROS) detoxification enzymes were also observed in these transgenic lines. Thus, based on our data and previous studies, it was hypothesized that constitutive overexpression of the GmGlb1-1 gene can interfere in the dynamics of ROS production and NO scavenging, enhancing the acquired systemic acclimation to biotic and abiotic stresses, and improving the cellular homeostasis. Therefore, these collective data suggest that ectopic or nematode-induced overexpression, or enhanced expression of the GmGlb1-1 gene using CRISPR/dCas9 offers great potential for application in commercial soybean cultivars aiming to reduce plant susceptibility to M. incognita.
MAIN CONCLUSION: The overexpression of the GmGlb1-1 gene reduces plant susceptibility to Meloidogyne incognita. Non-symbiotic globin class #1 (Glb1) genes are expressed in different plant organs, have a high affinity for oxygen, and are related to nitric oxide (NO) turnover. Previous studies showed that soybean Glb1 genes are upregulated in soybean plants under flooding conditions. Herein, the GmGlb1-1 gene was identified in soybean as being upregulated in the nematode-resistant genotype PI595099 compared to the nematode-susceptible cultivar BRS133 during plant parasitism by Meloidogyne incognita. The Arabidopsis thaliana and Nicotiana tabacum transgenic lines overexpressing the GmGlb1-1 gene showed reduced susceptibility to M. incognita. Consistently, gall morphology data indicated that pJ2 nematodes that infected the transgenic lines showed developmental alterations and delayed parasitism progress. Although no significant changes in biomass and seed yield were detected, the transgenic lines showed an elongated, etiolation-like growth under well-irrigation, and also developed more axillary roots under flooding conditions. In addition, transgenic lines showed upregulation of some important genes involved in plant defense response to oxidative stress. In agreement, higher hydrogen peroxide accumulation and reduced activity of reactive oxygen species (ROS) detoxification enzymes were also observed in these transgenic lines. Thus, based on our data and previous studies, it was hypothesized that constitutive overexpression of the GmGlb1-1 gene can interfere in the dynamics of ROS production and NO scavenging, enhancing the acquired systemic acclimation to biotic and abiotic stresses, and improving the cellular homeostasis. Therefore, these collective data suggest that ectopic or nematode-induced overexpression, or enhanced expression of the GmGlb1-1 gene using CRISPR/dCas9 offers great potential for application in commercial soybean cultivars aiming to reduce plant susceptibility to M. incognita.
Authors: Pierre Abad; Jérôme Gouzy; Jean-Marc Aury; Philippe Castagnone-Sereno; Etienne G J Danchin; Emeline Deleury; Laetitia Perfus-Barbeoch; Véronique Anthouard; François Artiguenave; Vivian C Blok; Marie-Cécile Caillaud; Pedro M Coutinho; Corinne Dasilva; Francesca De Luca; Florence Deau; Magali Esquibet; Timothé Flutre; Jared V Goldstone; Noureddine Hamamouch; Tarek Hewezi; Olivier Jaillon; Claire Jubin; Paola Leonetti; Marc Magliano; Tom R Maier; Gabriel V Markov; Paul McVeigh; Graziano Pesole; Julie Poulain; Marc Robinson-Rechavi; Erika Sallet; Béatrice Ségurens; Delphine Steinbach; Tom Tytgat; Edgardo Ugarte; Cyril van Ghelder; Pasqua Veronico; Thomas J Baum; Mark Blaxter; Teresa Bleve-Zacheo; Eric L Davis; Jonathan J Ewbank; Bruno Favery; Eric Grenier; Bernard Henrissat; John T Jones; Vincent Laudet; Aaron G Maule; Hadi Quesneville; Marie-Noëlle Rosso; Thomas Schiex; Geert Smant; Jean Weissenbach; Patrick Wincker Journal: Nat Biotechnol Date: 2008-07-27 Impact factor: 54.908
Authors: Muhammad A Ali; Farrukh Azeem; Amjad Abbas; Faiz A Joyia; Hongjie Li; Abdelfattah A Dababat Journal: Front Plant Sci Date: 2017-05-09 Impact factor: 5.753
Authors: Marcos Fernando Basso; Paulo Cavalcanti Gomes Ferreira; Adilson Kenji Kobayashi; Frank G Harmon; Alexandre Lima Nepomuceno; Hugo Bruno Correa Molinari; Maria Fatima Grossi-de-Sa Journal: Plant Biotechnol J Date: 2019-06-14 Impact factor: 9.803