| Literature DB >> 36107345 |
Xuelin Jin1, Y Tehee Kim2, Kyubong Jo3.
Abstract
DNA binding fluorescent proteins are a powerful tool for single-molecule visualization. In this chapter, we discuss a protocol for the synthesis of DNA binding fluorescent proteins and visualization of single DNA molecules. This chapter includes stepwise methods for molecular cloning, reversible staining, two-color staining, sequence-specific staining, and microscopic visualization of single DNA molecules in a microfluidic device. This content will be useful for DNA characterization using DNA binding fluorescent proteins and its visualization at the single-molecule level.Entities:
Keywords: Characteristics; DNA binding fluorescent protein; Microfluidic device; Sequence-specific staining; Single-molecule observation
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Year: 2023 PMID： 36107345 DOI： 10.1007/978-1-0716-2667-2_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745