Literature DB >> 36107344

Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions.

Kara L Schneider1, Lucas E Reibenspies1,2, Thomas Nyström1, Sviatlana Shashkova3,4.   

Abstract

Fluorescent proteins within fluorescent fusions have been reported to affect cellular growth fitness via altering native protein function and intracellular localization. Here we report in detail a procedure to analyze the growth characteristics of yeast cells expressing such fusions in comparison to unmodified parental strain. This approach can serve as an initial step in fluorescent protein characterization in vivo.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Fluorescence; Growth curve; Protein fusion; Yeast

Mesh：

Substances：

Year:  2023        PMID： 36107344     DOI： 10.1007/978-1-0716-2667-2_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

1.  Cti6, a PHD domain protein, bridges the Cyc8-Tup1 corepressor and the SAGA coactivator to overcome repression at GAL1.

Authors:  Manolis Papamichos-Chronakis; Theodoros Petrakis; Eleni Ktistaki; Irini Topalidou; Dimitris Tzamarias
Journal:  Mol Cell       Date:  2002-06       Impact factor: 17.970

2.  Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.

Authors:  O SHIMOMURA; F H JOHNSON; Y SAIGA
Journal:  J Cell Comp Physiol       Date:  1962-06

3.  Polyglutamine toxicity in yeast uncovers phenotypic variations between different fluorescent protein fusions.

Authors:  Yuwei Jiang; Sonja E Di Gregorio; Martin L Duennwald; Patrick Lajoie
Journal:  Traffic       Date:  2016-11-01       Impact factor: 6.215

4.  Assessment of GFP Tag Position on Protein Localization and Growth Fitness in Yeast.

Authors:  Uri Weill; Gat Krieger; Zohar Avihou; Ron Milo; Maya Schuldiner; Dan Davidi
Journal:  J Mol Biol       Date:  2018-12-12       Impact factor: 5.469

5.  Cti6 is an Rpd3-Sin3 histone deacetylase-associated protein required for growth under iron-limiting conditions in Saccharomyces cerevisiae.

Authors:  Sergi Puig; Miranda Lau; Dennis J Thiele
Journal:  J Biol Chem       Date:  2004-05-09       Impact factor: 5.157

6.  Primary structure of the Aequorea victoria green-fluorescent protein.

Authors:  D C Prasher; V K Eckenrode; W W Ward; F G Prendergast; M J Cormier
Journal:  Gene       Date:  1992-02-15       Impact factor: 3.688

7.  Transcription factor clusters regulate genes in eukaryotic cells.

Authors:  Adam Jm Wollman; Sviatlana Shashkova; Erik G Hedlund; Rosmarie Friemann; Stefan Hohmann; Mark C Leake
Journal:  Elife       Date:  2017-08-25       Impact factor: 8.140

8.  Kar2p availability defines distinct forms of endoplasmic reticulum stress in living cells.

Authors:  Patrick Lajoie; Robyn D Moir; Ian M Willis; Erik L Snapp
Journal:  Mol Biol Cell       Date:  2012-01-04       Impact factor: 4.138

9.  A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.

Authors:  Nathan C Shaner; Gerard G Lambert; Andrew Chammas; Yuhui Ni; Paula J Cranfill; Michelle A Baird; Brittney R Sell; John R Allen; Richard N Day; Maria Israelsson; Michael W Davidson; Jiwu Wang
Journal:  Nat Methods       Date:  2013-03-24       Impact factor: 28.547

10.  PRECOG: a tool for automated extraction and visualization of fitness components in microbial growth phenomics.

Authors:  Luciano Fernandez-Ricaud; Olga Kourtchenko; Martin Zackrisson; Jonas Warringer; Anders Blomberg
Journal:  BMC Bioinformatics       Date:  2016-06-23       Impact factor: 3.169
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