Stylianos Mastronikolis1,2, Evangelos Tsiambas3, Marina Pagkalou4, Olga E Makri1, Vasiliki K Thomopoulou5, Dimitrios Peschos6, Vasileios Ragos7, Dimitrios Roukas8, Constantinos D Georgakopoulos1. 1. Department of Ophthalmology, Medical School, University of Patras, Patras, Greece. 2. Neurosurgery Department, James Cook University Hospital, Middlesbrough, U.K. 3. Department of Cytology, 417 VA Hospital (NIMTS), Athens, Greece; tsiambasecyto@yahoo.gr. 4. Department of Chemistry, University of Crete, Heraklion, Greece. 5. Department of Chemistry, University of Patras, Patras, Greece. 6. Department of Physiology, Medical School, University of Ioannina, Ioannina, Greece. 7. Department of Maxillofacial, Medical School, University of Ioannina, Ioannina, Greece. 8. Department of Psychiatry, 417 VA Hospital (NIMTS), Athens, Greece.
Abstract
BACKGROUND/AIM: Mechanisms of c-FOS activation in the onset and progression of pterygia remain under investigation. This study aimed to comparatively analyze c-FOS proto-oncogene expression levels in neoplastic pterygia and normal epithelia. MATERIALS AND METHODS: We used a liquid-based cytology assay on thirty (n=30) pterygia cell populations and normal epithelia (n=10) extracted by a smooth scraping of conjunctiva epithelia. Applying a cell spot-based technique, we constructed five (n=5) slides, each containing eight (n=8) cell spots. A modified immune-cytochemistry (ICC) assay for c-FOS protein was used. Additionally, digital image analysis was implemented to calculate c-FOS immunostaining intensity levels. RESULTS: High staining intensity levels of c-FOS were detected in 12/30 (40%), whereas the rest 18/30 (60%) demonstrated moderate expression. c-FOS levels were statistically significantly higher in the pterygia compared to control tissues (p=0.001). c-FOS levels in the pterygia were not associated with the sex of patients (p=0.678), the presence of recurrent lesion (p=0.390) or the location of the lesion (p=0.158). The levels of c-FOS, however, were marginally significantly affected by the morphology of the pterygia (p=0.005). More precisely, the c-FOS levels were significantly higher in pterygia with a fleshy morphology. CONCLUSION: c-FOS over-expression is observed frequently in pterygia. It seems to be critically involved in the molecular mechanism of the lesion by its over-expression affecting partially their morphological features. Cell spot liquid - based array analysis is an innovative, easy to implement technique for simultaneously evaluating a broad spectrum of molecules in multiple specimens on the same slide.
BACKGROUND/AIM: Mechanisms of c-FOS activation in the onset and progression of pterygia remain under investigation. This study aimed to comparatively analyze c-FOS proto-oncogene expression levels in neoplastic pterygia and normal epithelia. MATERIALS AND METHODS: We used a liquid-based cytology assay on thirty (n=30) pterygia cell populations and normal epithelia (n=10) extracted by a smooth scraping of conjunctiva epithelia. Applying a cell spot-based technique, we constructed five (n=5) slides, each containing eight (n=8) cell spots. A modified immune-cytochemistry (ICC) assay for c-FOS protein was used. Additionally, digital image analysis was implemented to calculate c-FOS immunostaining intensity levels. RESULTS: High staining intensity levels of c-FOS were detected in 12/30 (40%), whereas the rest 18/30 (60%) demonstrated moderate expression. c-FOS levels were statistically significantly higher in the pterygia compared to control tissues (p=0.001). c-FOS levels in the pterygia were not associated with the sex of patients (p=0.678), the presence of recurrent lesion (p=0.390) or the location of the lesion (p=0.158). The levels of c-FOS, however, were marginally significantly affected by the morphology of the pterygia (p=0.005). More precisely, the c-FOS levels were significantly higher in pterygia with a fleshy morphology. CONCLUSION: c-FOS over-expression is observed frequently in pterygia. It seems to be critically involved in the molecular mechanism of the lesion by its over-expression affecting partially their morphological features. Cell spot liquid - based array analysis is an innovative, easy to implement technique for simultaneously evaluating a broad spectrum of molecules in multiple specimens on the same slide.
Authors: G J Fisher; H S Talwar; J Lin; P Lin; F McPhillips; Z Wang; X Li; Y Wan; S Kang; J J Voorhees Journal: J Clin Invest Date: 1998-03-15 Impact factor: 14.808
Authors: Evangelos Tsiambas; Christos Riziotis; Nicholas S Mastronikolis; Dimitrios Peschos; Alexandros Mortakis; Grigorios Kyroysis; Stylianos N Mastronikolis; Anna Batistatou; Andreas C Lazaris; Efstratios Patsouris; Vasileios Ragos Journal: Anticancer Res Date: 2018-10 Impact factor: 2.480