Hajar Molaee1, Yahya Tahamtan2, Ehlam Saeednezhad3, Masoumeh Hayati4. 1. Immunology Department, Shiraz Branch, Agricultural Research, Razi Vaccine and Serum Research Institute, Education and Extension Organization (AREEO), Shiraz, Iran. 2. Microbiology Department, Shiraz Branch, Agricultural Research, Razi Vaccine and Serum Research Institute, Education and Extension Organization (AREEO), Shiraz, Iran. yahyatahamtan@yahoo.com. 3. Department of Microbiology Shiraz Branch, Islamic Azad University, Shiraz, Iran. 4. Molecullar Biology Department, Shiraz Branch, Agricultural Research, Razi Vaccine and Serum Research Institute, Education and Extension Organization (AREEO), Shiraz, Iran.
Abstract
BACKGROUND: Mannheimia haemolytica is one of the main agents of domestic pneumonic mannheimiosis, but a proper vaccine has not been explored in IRAN. METHODS AND RESULTS: 362 lung and nasal samples from sick domestic animal were detected by culture and PCR methods. Totally, 71 M. haemolytica isolates were identified in three main serotypes (A1, A2, and A6). Serotypes A2 (38/71; 54%) and A1 (25/71; 39%) were the most frequently detected, whereas the A6 serotype was detected with a frequency of less than 1% (1/71; 1%) and 7 isolates remained unknown (7/71; 10%). Subsequently, M. haemolytica vaccinal strain was developed and then formalin-killed vaccine was prepared. It provided the best protection against mannheimiosis in sheep which was proved by indirect ELISA. CONCLUSIONS: Our results suggest that the efficacy and safety of vaccine strain are remarkable and may serve as a new therapeutic target in mannheimiosis.
BACKGROUND: Mannheimia haemolytica is one of the main agents of domestic pneumonic mannheimiosis, but a proper vaccine has not been explored in IRAN. METHODS AND RESULTS: 362 lung and nasal samples from sick domestic animal were detected by culture and PCR methods. Totally, 71 M. haemolytica isolates were identified in three main serotypes (A1, A2, and A6). Serotypes A2 (38/71; 54%) and A1 (25/71; 39%) were the most frequently detected, whereas the A6 serotype was detected with a frequency of less than 1% (1/71; 1%) and 7 isolates remained unknown (7/71; 10%). Subsequently, M. haemolytica vaccinal strain was developed and then formalin-killed vaccine was prepared. It provided the best protection against mannheimiosis in sheep which was proved by indirect ELISA. CONCLUSIONS: Our results suggest that the efficacy and safety of vaccine strain are remarkable and may serve as a new therapeutic target in mannheimiosis.
Authors: Jason Gioia; Xiang Qin; Huaiyang Jiang; Kenneth Clinkenbeard; Reggie Lo; Yamei Liu; George E Fox; Shailaja Yerrapragada; Michael P McLeod; Thomas Z McNeill; Lisa Hemphill; Erica Sodergren; Qiaoyan Wang; Donna M Muzny; Farah J Homsi; George M Weinstock; Sarah K Highlander Journal: J Bacteriol Date: 2006-10 Impact factor: 3.490
Authors: Susanna C Roehrig; Hon Q Tran; Volker Spehr; Nikolas Gunkel; Paul M Selzer; Heinz J Ullrich Journal: Vet Microbiol Date: 2006-12-22 Impact factor: 3.293
Authors: Faez Firdaus Abdullah Jesse; Eric Lim Teik Chung; Yusuf Abba; Kalai Vaani Muniandy; Ahmad Hafizin Ahmad Ramizi Tan; Dharshini Maslamany; Asinamai Athliamai Bitrus; Mohd Azmi Mohd Lila; Mohd Jefri Norsidin Journal: Trop Anim Health Prod Date: 2018-08-07 Impact factor: 1.559
Authors: Rohana P Dassanayake; Sudarvili Shanthalingam; Caroline N Herndon; Paulraj K Lawrence; E Frances Cassirer; Kathleen A Potter; William J Foreyt; Kenneth D Clinkenbeard; Subramaniam Srikumaran Journal: Vet Microbiol Date: 2008-08-05 Impact factor: 3.293
Authors: Anthony W Confer; Sahlu Ayalew; Roger J Panciera; Marie Montelongo; Lisa C Whitworth; Jordan D Hammer Journal: Vaccine Date: 2003-06-20 Impact factor: 3.641