| Literature DB >> 36093380 |
Elena-Sofia Heinl1, Sebastian Lorenz1, Barbara Schmidt2, Nouf Nasser M Laqtom3, Joseph R Mazzulli4, Laetitia Francelle4, Timothy W Yu5,6, Benjamin Greenberg7, Stephan Storch8, Ines Tegtmeier1, Helga Othmen1,9, Katja Maurer1, Malin Steinfurth1, Ralph Witzgall9, Vladimir Milenkovic10, Christian H Wetzel10, Markus Reichold1.
Abstract
The SARS-CoV-2 virus has triggered a worldwide pandemic. According to the BioGrid database, CLN7 (MFSD8) is thought to interact with several viral proteins. The aim of this work was to investigate a possible involvement of CLN7 in the infection process. Experiments on a CLN7-deficient HEK293T cell line exhibited a 90% reduced viral load compared to wild-type cells. This observation may be linked to the finding that CLN7 ko cells have a significantly reduced GM1 content in their cell membrane. GM1 is found highly enriched in lipid rafts, which are thought to play an important role in SARS-CoV-2 infection. In contrast, overexpression of CLN7 led to an increase in viral load. This study provides evidence that CLN7 is involved in SARS-CoV-2 infection. This makes it a potential pharmacological target for drug development against COVID-19. Furthermore, it provides insights into the physiological function of CLN7 where still only little is known about.Entities:
Keywords: Cell biology; Virology
Year: 2022 PMID： 36093380 PMCID： PMC9444308 DOI： 10.1016/j.isci.2022.105082
Source DB: PubMed Journal: iScience ISSN： 2589-0042