Literature DB >> 36087255

Cell Death Analysis in Cancer Spheroids from a Microfluidic Device.

Julie Lafontaine1, Elena Refet-Mollof1,2, Ouafa Najyb1, Thomas Gervais1,2,3, Philip Wong4,5,6,7.   

Abstract

Microfluidic technology facilitates the generation of 3D spheroids from cancer cells, a more suitable model for preclinical therapeutic studies. This system opens the possibility to test many drugs combination at a low cost. Here we describe the use of microfluidic devices for cytotoxicity evaluation on cancer spheroids for the discovery of drugs that could be used in combination with radiotherapy. Device fabrication, preparation, and seeding are also covered. Cell death arising following treatment is detected and characterized according to spheroid size, colony formation assays, and flow cytometry analysis of apoptotic marker annexin V.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  3D culture; Annexin V; Chemotherapy; Clonogenic assay; Drug screening; ImageJ; Lab-on-chip; Microfluidic; Radiotherapy; Spheroids

Mesh：

Year:  2022        PMID： 36087255     DOI： 10.1007/978-1-0716-2553-8_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Monolithic microfabricated valves and pumps by multilayer soft lithography.

Authors:  M A Unger; H P Chou; T Thorsen; A Scherer; S R Quake
Journal:  Science       Date:  2000-04-07       Impact factor: 47.728

2.  Long-term three-dimensional cell culture and anticancer drug activity evaluation in a microfluidic chip.

Authors:  Karina Ziółkowska; Agnieszka Stelmachowska; Radosław Kwapiszewski; Michał Chudy; Artur Dybko; Zbigniew Brzózka
Journal:  Biosens Bioelectron       Date:  2012-06-19       Impact factor: 10.618

3.  Fluorescence hyperspectral imaging for live monitoring of multiple spheroids in microfluidic chips.

Authors:  Amélie St-Georges-Robillard; Mathieu Masse; Maxime Cahuzac; Mathias Strupler; Bishnubrata Patra; Adriana Mari Orimoto; Jennifer Kendall-Dupont; Benjamin Péant; Anne-Marie Mes-Masson; Frédéric Leblond; Thomas Gervais
Journal:  Analyst       Date:  2018-08-06       Impact factor: 4.616

4.  Paraffin-embedding lithography and micro-dissected tissue micro-arrays: tools for biological and pharmacological analysis of ex vivo solid tumors.

Authors:  Kayla Simeone; Robin Guay-Lord; Mohammad Abdul Lateef; Benjamin Péant; Jennifer Kendall-Dupont; Adriana Mari Orimoto; Euridice Carmona; Diane Provencher; Fred Saad; Thomas Gervais; Anne-Marie Mes-Masson
Journal:  Lab Chip       Date:  2019-02-12       Impact factor: 6.799

5.  Multi-size spheroid formation using microfluidic funnels.

Authors:  M Marimuthu; N Rousset; A St-Georges-Robillard; M A Lateef; M Ferland; A-M Mes-Masson; T Gervais
Journal:  Lab Chip       Date:  2018-01-16       Impact factor: 6.799

6.  X-ray on chip: Quantifying therapeutic synergies between radiotherapy and anticancer drugs using soft tissue sarcoma tumor spheroids.

Authors:  Maeva Bavoux; Yuji Kamio; Emmanuelle Vigneux-Foley; Julie Lafontaine; Ouafa Najyb; Elena Refet-Mollof; Jean-François Carrier; Thomas Gervais; Philip Wong
Journal:  Radiother Oncol       Date:  2021-01-29       Impact factor: 6.280

7.  Micro-dissected tumor tissues on chip: an ex vivo method for drug testing and personalized therapy.

Authors:  M Astolfi; B Péant; M A Lateef; N Rousset; J Kendall-Dupont; E Carmona; F Monet; F Saad; D Provencher; A-M Mes-Masson; T Gervais
Journal:  Lab Chip       Date:  2016-01-21       Impact factor: 6.799

8.  Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis.

Authors:  G Koopman; C P Reutelingsperger; G A Kuijten; R M Keehnen; S T Pals; M H van Oers
Journal:  Blood       Date:  1994-09-01       Impact factor: 22.113

9.  Action of x-rays on mammalian cells.

Authors:  T T PUCK; P I MARCUS
Journal:  J Exp Med       Date:  1956-05-01       Impact factor: 14.307
  9 in total

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