Literature DB >> 36083559

High-Throughput Binder Confirmation (HTBC).

Bing Xia1, Jennifer D Summerfield2.   

Abstract

In this chapter, we describe a DEL "recipe" approach to hit confirmation using on-DNA cleavable linkers which can be followed by direct AS-MS evaluation and identification of binder(s) from the released small-molecule mixture. When coupled with automation, this platform creates opportunity for enabling an even higher-throughput binder confirmation (HTBC) platform to allow full interrogation of selections results. Two on-DNA cleavable strategies of releasing small molecules from DNA are reported herein.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  DNA-encoded library (DEL); High-throughput confirmation; Hit confirmation; Photocleavable linker (PCL); Tetrahydropyranyl cleavable linker (THP)

Mesh：

Substances：

Year:  2022        PMID： 36083559     DOI： 10.1007/978-1-0716-2545-3_24

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Design, synthesis and selection of DNA-encoded small-molecule libraries.

Authors:  Matthew A Clark; Raksha A Acharya; Christopher C Arico-Muendel; Svetlana L Belyanskaya; Dennis R Benjamin; Neil R Carlson; Paolo A Centrella; Cynthia H Chiu; Steffen P Creaser; John W Cuozzo; Christopher P Davie; Yun Ding; G Joseph Franklin; Kurt D Franzen; Malcolm L Gefter; Steven P Hale; Nils J V Hansen; David I Israel; Jinwei Jiang; Malcolm J Kavarana; Michael S Kelley; Christopher S Kollmann; Fan Li; Kenneth Lind; Sibongile Mataruse; Patricia F Medeiros; Jeffrey A Messer; Paul Myers; Heather O'Keefe; Matthew C Oliff; Cecil E Rise; Alexander L Satz; Steven R Skinner; Jennifer L Svendsen; Lujia Tang; Kurt van Vloten; Richard W Wagner; Gang Yao; Baoguang Zhao; Barry A Morgan
Journal:  Nat Chem Biol       Date:  2009-08-02       Impact factor: 15.040

2.  A single-bead decode strategy using electrospray ionization mass spectrometry and a new photolabile linker: 3-amino-3-(2-nitrophenyl)propionic acid.

Authors:  B B Brown; D S Wagner; H M Geysen
Journal:  Mol Divers       Date:  1995-09       Impact factor: 2.943

3.  DNA-Encoded Library Hit Confirmation: Bridging the Gap Between On-DNA and Off-DNA Chemistry.

Authors:  Bing Xia; G Joseph Franklin; Xiaojie Lu; Katie L Bedard; LaShadric C Grady; Jennifer D Summerfield; Eric X Shi; Bryan W King; Kenneth E Lind; Cynthia Chiu; Eleanor Watts; Vera Bodmer; Xiaopeng Bai; Lisa A Marcaurelle
Journal:  ACS Med Chem Lett       Date:  2021-06-03       Impact factor: 4.632
  3 in total

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