Macrophages, macrophage foam cells, and eccentric intimal thickening in the coronary arteries of young children.

We surveyed the incidence and location of macrophages and macrophage foam cells in the coronary artery intima of 63 children that died in the first 5 years of life. We related the data on macrophages and macrophage foam cells to intimal smooth muscle cells and to measurements of intima:media area and thickness. All morphometric data were obtained from coronary arteries that were fixed by perfusion with glutaraldehyde under pressure, embedded in Maraglas, and cut into 1-micron cross-sections, and 65-nm fine sections. Coronary artery intima was always thicker (eccentric thickening) at bifurcations in the half of the circumference opposite to the flow divider. This was true for both male and female children. The remaining part of the coronary artery intima was less thick (diffuse thickening). Both types of intimal thickening were composed of an inner layer in which glycosaminoglycan ground substance predominated and a deeper musculoelastic layer. Fifty-nine children (94%) had intimal macrophages. Twenty children also had macrophage foam cells. Of 33 children aged to 8 months, 15 (45%) had macrophage foam cells. Of the 30 children older than 8 months, 5 (17%) had macrophage foam cells. Macrophages and macrophage foam cells occurred in the GAG-rich layer of the intima as isolated cells. In 5 infants macrophage foam cells occurred also as clusters of many cells. Macrophages were more numerous in cases that also had macrophage foam cells. Macrophages were 6 times, and macrophage foam cells 5 times more numerous in eccentric intimal thickening than in diffuse intimal thickening.