Literature DB >> 36066715

Telomere Aberration Detection by PNA FISH Probe.

Takamitsu A Kato1.   

Abstract

Telomere is a structure of the end cap of chromosomes. Telomere gets shorter as cell aging and progressing cell division. Shorter telomere may cause telomere fusion, thus inducing genomic instability. Telomere dysfunction can be visualized by PNA FISH probe against telomere repeat sequence (TTAGGG)n. PNA probes have higher hybridization affinity than DNA probes. The traditional FISH or modified FISH protocol can stain telomere relatively easier than whole-chromosome painting probes. This chapter introduces PNA telomere FISH protocol to visualize telomere signals on metaphase chromosomes.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  End cap of chromosomes; Heyflick’s limit; PNA FISH; Telomere; Telomere fusion; Telomeric repeat sequences

Mesh：

Substances：

Year:  2023        PMID： 36066715     DOI： 10.1007/978-1-0716-2433-3_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

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Journal:  Science       Date:  2001-09-28       Impact factor: 47.728

8.  Evidence for an alternative mechanism for maintaining telomere length in human tumors and tumor-derived cell lines.

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Journal:  Nat Med       Date:  1997-11       Impact factor: 53.440

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Journal:  Science       Date:  1991-12-06       Impact factor: 47.728

10.  Genomic instability and telomere fusion of canine osteosarcoma cells.

Authors:  Junko Maeda; Charles R Yurkon; Hiroshi Fujisawa; Masami Kaneko; Stefan C Genet; Erica J Roybal; Garrett W Rota; Ethan R Saffer; Barbara J Rose; William H Hanneman; Douglas H Thamm; Takamitsu A Kato
Journal:  PLoS One       Date:  2012-08-16       Impact factor: 3.240
  10 in total

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