Literature DB >> 3605614

Enzymatic determination of the branched-chain alpha-keto acids.

G W Goodwin, M J Kuntz, R Paxton, R A Harris.   

Abstract

A spectrophotometric endpoint assay for determination of branched-chain alpha-keto acids is described. The assay depends on measurement of the NADH produced after addition of branched-chain alpha-keto acid dehydrogenase. Interference by pyruvate and alpha-ketobutyrate was eliminated by pretreating the sample with pyruvate dehydrogenase. The method yielded a peripheral venous plasma value of 59 +/- 5 microM (mean +/- SE) for the branched-chain alpha-keto acids of five overnight fasted healthy humans.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3605614     DOI： 10.1016/0003-2697(87)90430-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Production of recombinant E1 component of branched-chain alpha-keto acid dehydrogenase complex.

Authors:  J W Hawes; Y Zhao; K M Popov; Y Shimomura; R A Harris
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

2.  Gender difference in regulation of branched-chain amino acid catabolism.

Authors:  R Kobayashi; Y Shimomura; T Murakami; N Nakai; N Fujitsuka; M Otsuka; N Arakawa; K M Popov; R A Harris
Journal:  Biochem J       Date:  1997-10-15       Impact factor: 3.857

3.  Protection by pyruvate against glutamate neurotoxicity is mediated by astrocytes through a glutathione-dependent mechanism.

Authors:  Yifeng Miao; Yongming Qiu; Yuchang Lin; Zengli Miao; Jing Zhang; Xiaojie Lu
Journal:  Mol Biol Rep       Date:  2010-02-24       Impact factor: 2.316

4.  Longer-term regulation of branched-chain-2-oxoacid dehydrogenase complex studied in rat hepatocytes in culture.

Authors:  M Beggs; J M Shaw; P J Randle
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.