| Literature DB >> 36040606 |
Renata Mazurek1, Kiyotake Ishikawa2.
Abstract
Formation of neutralizing antibodies and cellular immune response with repeat adeno-associated virus (AAV) gene therapy dosing are critical concerns in translational, large animal studies. The enzyme-linked immunospot/immunosorbent spot (ELISpot) assay introduced a way to track B- and/or T-cell response to therapy over time at a protein level. We describe the protocol for this assay looking at relative interferon (IFN)-γ secretion in pre- and post-AAV injections in a pig model.Entities:
Keywords: ELISpot assay; Gene therapy; Interferon-γ; Large animal; Peripheral blood mononuclear cells
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Year: 2022 PMID： 36040606 DOI： 10.1007/978-1-0716-2707-5_25
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745