Ahmad Rayyan1, Amber Ather2, Kenneth Hargreaves1, Nikita B Ruparel3. 1. Department of Endodontics, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229. 2. Department of Endodontics, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229; Department of Endodontics, Virginia Commonwealth University, 520 North 12th Street, Box 980566, Richmond, Virginia 23298-0566. 3. Department of Endodontics, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229. Electronic address: ruparel@uthscsa.edu.
Abstract
INTRODUCTION: Aerosol generation in a dental setting is a critical concern and approaches that aim at decreasing bacterial load in aerosols is of high priority for dental professionals. The objectives of this study were to evaluate the relative effect of various endodontic procedures on the generation and dissemination of aerosols and the effect of 0.1% sodium hypochlorite (NaOCl) in dental unit waterlines (DUWLs) on the bacterial load in the generated aerosols in a clinical setting. METHODS: The study was completed in two phases. The classical passive sampling technique using brain heart infusion agar plates was utilized. Agar plates were strategically placed throughout the operatory at pre-defined locations. PHASE I: To evaluate the effect of different endodontic procedures on generation and dissemination of aerosols, we collected a total of 38 samples. Following baseline collection, test samples were collected during vital pulp therapy (VPT) full pulpotomy (n=10), non-surgical root canal therapy (NSRCT; n=10), surgical root canal therapy (SRCT; n=10), and incision and drainage (I&D; n=8) procedures. Bacterial growth was expressed as colony-forming units at 48 hours post-sample collection. Data were analyzed using 1-way analysis of variance with Tukey's multiple comparisons post-hoc test. PHASE II: To evaluate the effect of 0.1% NaOCl in DUWL on the bacterial load in the generated aerosols, a total of 30 samples were collected. All procedures including VPT (n=10), NSRCT (n=10), and SRCT (n=10) were performed with 0.1% NaOCl in DUWL. Bacterial growth was expressed as colony-forming units at 48 hours post-sample collection. Data were analyzed using 2-way analysis of variance with Tukey's multiple comparisons post-hoc test. RESULTS: All endodontic procedures generated aerosols at all tested locations except I&D. Aerosols were disseminated as far as 3m from the patient's head with no significant difference between various locations (p>0.05). VPT procedures generated the maximum number of aerosols compared to NSRCT and SRCT. Adding 0.1% NaOCl to DUWLs significantly reduced the bacterial load in the generated aerosols in all treatment groups compared to groups treated with untreated waterlines (p<0.05). No significant difference was noted in the bacterial load between all groups with treated waterlines (p>0.05). CONCLUSIONS: All tested endodontic procedures led to the generation and dissemination of contaminated aerosols, and the addition of 0.1% NaOCl as a biocide to the DUWL led to a statistically significant reduction in the bacterial load.
INTRODUCTION: Aerosol generation in a dental setting is a critical concern and approaches that aim at decreasing bacterial load in aerosols is of high priority for dental professionals. The objectives of this study were to evaluate the relative effect of various endodontic procedures on the generation and dissemination of aerosols and the effect of 0.1% sodium hypochlorite (NaOCl) in dental unit waterlines (DUWLs) on the bacterial load in the generated aerosols in a clinical setting. METHODS: The study was completed in two phases. The classical passive sampling technique using brain heart infusion agar plates was utilized. Agar plates were strategically placed throughout the operatory at pre-defined locations. PHASE I: To evaluate the effect of different endodontic procedures on generation and dissemination of aerosols, we collected a total of 38 samples. Following baseline collection, test samples were collected during vital pulp therapy (VPT) full pulpotomy (n=10), non-surgical root canal therapy (NSRCT; n=10), surgical root canal therapy (SRCT; n=10), and incision and drainage (I&D; n=8) procedures. Bacterial growth was expressed as colony-forming units at 48 hours post-sample collection. Data were analyzed using 1-way analysis of variance with Tukey's multiple comparisons post-hoc test. PHASE II: To evaluate the effect of 0.1% NaOCl in DUWL on the bacterial load in the generated aerosols, a total of 30 samples were collected. All procedures including VPT (n=10), NSRCT (n=10), and SRCT (n=10) were performed with 0.1% NaOCl in DUWL. Bacterial growth was expressed as colony-forming units at 48 hours post-sample collection. Data were analyzed using 2-way analysis of variance with Tukey's multiple comparisons post-hoc test. RESULTS: All endodontic procedures generated aerosols at all tested locations except I&D. Aerosols were disseminated as far as 3m from the patient's head with no significant difference between various locations (p>0.05). VPT procedures generated the maximum number of aerosols compared to NSRCT and SRCT. Adding 0.1% NaOCl to DUWLs significantly reduced the bacterial load in the generated aerosols in all treatment groups compared to groups treated with untreated waterlines (p<0.05). No significant difference was noted in the bacterial load between all groups with treated waterlines (p>0.05). CONCLUSIONS: All tested endodontic procedures led to the generation and dissemination of contaminated aerosols, and the addition of 0.1% NaOCl as a biocide to the DUWL led to a statistically significant reduction in the bacterial load.