Xin-Chao Liu1, Qi Ren1, Jie Guo2, Dong-Qian Chen1, Qiao-Qiao Li1, Xin-Yu Luo1, You-Fang Gu1, Wen-Chao Li3. 1. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang, Chuzhou, 233100, China. 2. Animal Husbandry Development Center of Lu'an City, Lu'an, 237000, China. 3. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang, Chuzhou, 233100, China. liwen303@126.com.
Abstract
BACKGROUND: Protozoans of Entamoeba spp. are one of the most common enteric parasites that infect humans and diverse animals including deer. PURPOSE: However, data regarding the prevalence and species/genotypes of Entamoeba spp. in deer in China is scarce. This study investigated the prevalence and species distribution of Entamoeba spp. in sika deer (Cervus nippon) in Anhui Province. METHODS: In our survey, 336 fecal samples were collected from five sika deer farms in different regions of Anhui Province. All samples were examined for the presence of Entamoeba spp. by PCR and phylogenetic analysis of the conserved 18S rRNA gene. RESULTS: 106/336 (31.5%) fecal samples were positive for Entamoeba spp. A statistically significant difference in the prevalence of Entamoeba spp. infection was observed between sampling farms (p < 0.001), and the prevalence of Entamoeba spp. in male and female sika deer showed no significant difference (p > 0.05). Sequence and phylogenetic analysis revealed the single species, E. bovis, was identified in this study. CONCLUSION: This is the first report about the identification of E. bovis in farm-raised sika deer in China, and these results expand our understanding of host range and species distribution of Entamoeba spp. in ruminants.
BACKGROUND: Protozoans of Entamoeba spp. are one of the most common enteric parasites that infect humans and diverse animals including deer. PURPOSE: However, data regarding the prevalence and species/genotypes of Entamoeba spp. in deer in China is scarce. This study investigated the prevalence and species distribution of Entamoeba spp. in sika deer (Cervus nippon) in Anhui Province. METHODS: In our survey, 336 fecal samples were collected from five sika deer farms in different regions of Anhui Province. All samples were examined for the presence of Entamoeba spp. by PCR and phylogenetic analysis of the conserved 18S rRNA gene. RESULTS: 106/336 (31.5%) fecal samples were positive for Entamoeba spp. A statistically significant difference in the prevalence of Entamoeba spp. infection was observed between sampling farms (p < 0.001), and the prevalence of Entamoeba spp. in male and female sika deer showed no significant difference (p > 0.05). Sequence and phylogenetic analysis revealed the single species, E. bovis, was identified in this study. CONCLUSION: This is the first report about the identification of E. bovis in farm-raised sika deer in China, and these results expand our understanding of host range and species distribution of Entamoeba spp. in ruminants.
Authors: Alison S Jacob; Eloise J Busby; Abigail D Levy; Natasha Komm; C Graham Clark Journal: J Eukaryot Microbiol Date: 2015-07-23 Impact factor: 3.346
Authors: C Graham Clark; Farrokh Kaffashian; Blessing Tawari; Jeffrey J Windsor; Anke Twigg-Flesner; Mina C G Davies-Morel; Joerg Blessmann; Frank Ebert; Babett Peschel; An Le Van; Colin J Jackson; Lorna Macfarlane; Egbert Tannich Journal: Int J Syst Evol Microbiol Date: 2006-09 Impact factor: 2.747
Authors: Jose L Huaman; Carlo Pacioni; Lily Kenchington-Evans; Mark Doyle; Karla J Helbig; Teresa G Carvalho Journal: Front Cell Infect Microbiol Date: 2022-06-10 Impact factor: 6.073