| Literature DB >> 36017801 |
Rabia Johnson1,2, Noluxabiso Mangwana1, Jyoti R Sharma1,2, Christo J F Muller1,2,3, Kholofelo Malemela1, Funanani Mashau1, Stephanie Dias1, Pritika Ramharack1,4, Craig Kinnear5,6, Brigitte Glanzmann4, Amsha Viraragavan5, Johan Louw1,3, Swastika Surujlal-Naicker7, Sizwe Nkambule8, Candice Webster9, Mongezi Mdhluli10, Glenda Gray11, Angela Mathee9, Wolfgang Preiser12, Alvera Vorster13, Shareefa Dalvie1,14, Renee Street8.
Abstract
This study was one of the first to detect Omicron sublineages BA.4 and BA.5 in wastewater from South Africa. Spearman rank correlation analysis confirmed a strong positive correlation between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA in wastewater samples and clinical cases (r = 0.7749, P < .0001). SARS-CoV-2 viral load detected in wastewater, resulting from the Delta-driven third wave, was significantly higher than during the Omicron-driven fourth wave. Whole-genome sequencing confirmed presence of Omicron lineage defining mutations in wastewater with the first occurrence reported 23 November 2021 (BA.1 predominant). The variant spread rapidly, with prevalence of Omicron-positive wastewater samples rising to >80% by 10 January 2022 with BA.2 as the predominant sublineage by 10 March 2022, whilst on 18 April 2022 BA.4 and BA.5 were detected in selected wastewater sites. These findings demonstrate the value of wastewater-based epidemiology to monitor the spatiotemporal spread and potential origin of new Omicron sublineages.Entities:
Keywords: B.1.5.9 (Omicron); B.1.617.2 (Delta) lineages; BA.1; BA.2; BA.3; SARS-CoV-2; and BA.5; genotyping; wastewater-based epidemiology
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Year: 2022 PMID： 36017801 PMCID： PMC9574669 DOI： 10.1093/infdis/jiac356
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 7.759