Relative genotoxicity of trichloroacetic acid (TCA) as revealed by different cytogenetic assays: bone marrow chromosome aberration, micronucleus and sperm-head abnormality in the mouse.

Trichloroacetic acid (TCA) has been tested for mutagenicity in a mouse in vivo system. Three different cytogenetic assays--bone marrow chromosomal aberrations, micronucleus and sperm-head abnormalities--have been carried out. Swiss mice have been treated with the chemical, administered via different routes (i.p. and p.o.), and in three acute and/or fractionated doses (5 consecutive daily) equivalent to the highest acute dose, and their cells sampled at different intervals. A variety of anomalies, occurring in higher percentages compared to controls, was observed in all cases. Comparison between single and fractionated dosing revealed the single dosing to be more effective cytogenetically. The results were route and time-dependent but not dose-responsive (exclusive of gaps). The relative sensitivity of the assays has been found to be: chromosome aberration greater than sperm-head abnormality greater than micronucleus. The results revealed the genotoxic property of TCA in the present test system.