Naveed Munir1, Zahed Mehmood2, Muhammad Shahid3, Samina Aslam4, Mazhar Abbas5, Huma Mehboob6, Samiah H Al-Mijallia7, Muhammad Jahangeer8, Qurrat Ul Ain Badar9. 1. Department of Biomedical Lab Sciences, School of Health Sciences, University of Management and Technology, Lahore, Pakistan. 2. Department of Biochemistry, Government College University, Faisalabad, Pakistan. 3. Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan. 4. Department of Chemistry, The Women University Multan, Pakistan. 5. Department of Biochemistry, University of Veterinary and Animals Science Lahore (Jhang Campus), Jhang, Pakistan. 6. Department of Biochemistry, Government Women College University Faisalabad, Pakistan. 7. Department of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. 8. Food and Biotechnology Research Center, Pakistan Council of Scientific and Industrial Research (PCSIR), Laboratories Complex Lahore, Pakistan. 9. School of Biochemistry and Biotechnology University of the Punjab, Lahore, Pakistan.
Abstract
Background: Natural medicines are being used for the treatment of various disorders due to pharmacological, therapeutical, and nutraceuticals characteristics. Objectives: Current research was planned to explore In vitro pharmacological response of phytochemical constituents extracted from C. monnieri' seeds using aqueous ethanol (70%). Methods: Qualitative and quantitative measurements for phytochemical constituents were performed following reference protocols. Then In vitro antioxidant potential, cytotoxic studies, antimicrobial, and spermicidal pharmacological response of C. monnieri extract were investigated. Results: The results of High Performance Liquid Chromatography (HPLC), Fourier Transform Infra-Red (FTIR) spectroscopy, and Atomic Absorption Spectrophotometer (AAS) explored the presence of wide range of bioactive compounds with significant (p<.05) antioxidant activities. Cytotoxic studies revealed significant (p<.05) protective behavior of C. monnieri evaluated using CtDNA damage protection, against Salmonella typhi TA98 and TA100, RBCs membrane stabilizing and clot lysis assay. It was also found that selected herb has antibacterial and antifungal activities. The results of spermicidal study on human (n = 30) spermatozoa revealed significant (p<.05) contraceptive per vaginal behavior of this natural medicinal plant. Conclusion: It could be concluded that C. monnieri showed significant pharmacological activities with non-toxic behavior, however In vivo study in animals and clinical trials are required to declare this natural herb as therapeutic agent.
Background: Natural medicines are being used for the treatment of various disorders due to pharmacological, therapeutical, and nutraceuticals characteristics. Objectives: Current research was planned to explore In vitro pharmacological response of phytochemical constituents extracted from C. monnieri' seeds using aqueous ethanol (70%). Methods: Qualitative and quantitative measurements for phytochemical constituents were performed following reference protocols. Then In vitro antioxidant potential, cytotoxic studies, antimicrobial, and spermicidal pharmacological response of C. monnieri extract were investigated. Results: The results of High Performance Liquid Chromatography (HPLC), Fourier Transform Infra-Red (FTIR) spectroscopy, and Atomic Absorption Spectrophotometer (AAS) explored the presence of wide range of bioactive compounds with significant (p<.05) antioxidant activities. Cytotoxic studies revealed significant (p<.05) protective behavior of C. monnieri evaluated using CtDNA damage protection, against Salmonella typhi TA98 and TA100, RBCs membrane stabilizing and clot lysis assay. It was also found that selected herb has antibacterial and antifungal activities. The results of spermicidal study on human (n = 30) spermatozoa revealed significant (p<.05) contraceptive per vaginal behavior of this natural medicinal plant. Conclusion: It could be concluded that C. monnieri showed significant pharmacological activities with non-toxic behavior, however In vivo study in animals and clinical trials are required to declare this natural herb as therapeutic agent.
Authors: Muhammad Akram; Muhammad Riaz; Naveed Munir; Akhtar Rasul; Muhammad Daniyal; Syed Muhammad Ali Shah; Mohammad Ali Shariati; Ghazala Shaheen; Naheed Akhtar; Farzana Parveen; Naheed Akhter; Aymen Owais Ghauri; Abdul Wadood Chishti; Muhammad Usman Sarwar; Fahad Said Khan Journal: Clin Exp Pharmacol Physiol Date: 2020-03-15 Impact factor: 2.557
Authors: Muhammad Imran; Nasir Rasool; Komal Rizwan; Muhammad Zubair; Muhammad Riaz; Muhammad Zia-Ul-Haq; Usman Ali Rana; Ayman Nafady; Hawa Ze Jaafar Journal: Chem Cent J Date: 2014-02-13 Impact factor: 4.215