Xinpei Han1, Yadi Xing2,3, Yaqian Zhu4,5, Lei Luo4,5, Lulu Liu4,5, Yaohua Zhai4,5, Wenjing Wang4,5, Ruixing Shao1, Maozhi Ren4,5, Fuguang Li6,7, Qinghua Yang8. 1. College of Agronomy, Henan Agricultural University, Zhengzhou, China. 2. Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China. xingyadi@zzu.edu.cn. 3. Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China. xingyadi@zzu.edu.cn. 4. Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China. 5. Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China. 6. Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China. aylifug@caas.cn. 7. Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China. aylifug@caas.cn. 8. College of Agronomy, Henan Agricultural University, Zhengzhou, China. yangqh2000@163.com.
Abstract
MAIN CONCLUSION: GhMYC2 regulates the gossypol biosynthesis pathway in cotton through activation of the expression of gossypol synthesis gene CYP71BE79, CDNC, CYP706B1, DH1, and CYP82D113. Cotton is one of the main cash crops globally. Cottonseed contains fiber, fat, protein, and starch, and has important economic value. However, gossypol in cottonseed seriously affects the development and utilization of cottonseed. Nonetheless, gossypol has great application potential in agriculture, medicine, and industry. Therefore, it is very important to study gossypol biosynthesis and its upstream regulatory pathways. It has been reported that the content of gossypol in hairy roots of cotton is regulated through jasmonic acid signaling; however, the specific molecular mechanism has not been revealed yet. We found that the expression of basic helix-loop-helix family transcription factor GhMYC2 was significantly upregulated after exogenous administration of methyl jasmonate to cotton seedlings, and the content of gossypol changed significantly with the variation of GhMYC2 expression. Further studies revealed that GhMYC2 could specifically bind to the G-Box in the promoter region of CDNC, CYP706B1, DH1, CYP82D113, CYP71BE79 to activate its expression and regulate gossypol synthesis, and its activation of CYP71BE79 promoter was inhibited by GhJAZ2. Not only that GhMYC2 could also interact with GoPGF. In this work, the molecular mechanisms of gossypol biosynthesis regulated by GhMYC2 were analyzed. The results provide a theoretical basis for cultivating new varieties of low-gossypol or high-gossypol cotton and creating excellent germplasm resources.
MAIN CONCLUSION: GhMYC2 regulates the gossypol biosynthesis pathway in cotton through activation of the expression of gossypol synthesis gene CYP71BE79, CDNC, CYP706B1, DH1, and CYP82D113. Cotton is one of the main cash crops globally. Cottonseed contains fiber, fat, protein, and starch, and has important economic value. However, gossypol in cottonseed seriously affects the development and utilization of cottonseed. Nonetheless, gossypol has great application potential in agriculture, medicine, and industry. Therefore, it is very important to study gossypol biosynthesis and its upstream regulatory pathways. It has been reported that the content of gossypol in hairy roots of cotton is regulated through jasmonic acid signaling; however, the specific molecular mechanism has not been revealed yet. We found that the expression of basic helix-loop-helix family transcription factor GhMYC2 was significantly upregulated after exogenous administration of methyl jasmonate to cotton seedlings, and the content of gossypol changed significantly with the variation of GhMYC2 expression. Further studies revealed that GhMYC2 could specifically bind to the G-Box in the promoter region of CDNC, CYP706B1, DH1, CYP82D113, CYP71BE79 to activate its expression and regulate gossypol synthesis, and its activation of CYP71BE79 promoter was inhibited by GhJAZ2. Not only that GhMYC2 could also interact with GoPGF. In this work, the molecular mechanisms of gossypol biosynthesis regulated by GhMYC2 were analyzed. The results provide a theoretical basis for cultivating new varieties of low-gossypol or high-gossypol cotton and creating excellent germplasm resources.
Authors: Kalliopi Dodou; Rosaleen J Anderson; David A P Small; Paul W Groundwater Journal: Expert Opin Investig Drugs Date: 2005-11 Impact factor: 6.206
Authors: Chunpeng An; Lin Li; Qingzhe Zhai; Yanrong You; Lei Deng; Fangming Wu; Rong Chen; Hongling Jiang; Hang Wang; Qian Chen; Chuanyou Li Journal: Proc Natl Acad Sci U S A Date: 2017-10-02 Impact factor: 11.205