Nikola Stokovic1,2, Natalia Ivanjko1,2, Viktorija Rumenovic1,2, Anita Breski3, Kuber T Sampath4, Mihaela Peric2,5, Marko Pecina6, Slobodan Vukicevic7,8. 1. Laboratory for Mineralized Tissues, Center for Translational and Clinical Research, School of Medicine, University of Zagreb, Zagreb, Croatia. 2. Scientific Center of Excellence for Reproductive and Regenerative Medicine, Zagreb, Croatia. 3. Department of Pathology and Cytology, University Hospital Centre Zagreb, Zagreb, Croatia. 4. perForm Biologics Inc, Holliston, MA, USA. 5. Department for Intracellular Communication, Center for Translational and Clinical Research, School of Medicine, University of Zagreb, Zagreb, Croatia. 6. Department of Orthopaedic Surgery, School of Medicine, University of Zagreb, Zagreb, Croatia. 7. Laboratory for Mineralized Tissues, Center for Translational and Clinical Research, School of Medicine, University of Zagreb, Zagreb, Croatia. slobodan.vukicevic@mef.hr. 8. Scientific Center of Excellence for Reproductive and Regenerative Medicine, Zagreb, Croatia. slobodan.vukicevic@mef.hr.
Abstract
PURPOSE: Osteogrow, an osteoinductive device containing recombinant human Bone Morphogenetic Protein 6 (rhBMP6) in autologous blood coagulum, is a novel therapeutic solution for bone regeneration. This study aimed to evaluate different commercially available calcium phosphate synthetic ceramic particles as a compression-resistant matrix (CRM) added to Osteogrow implants to enhance their biomechanical properties. METHODS: Osteogrow implants with the addition of Vitoss, ChronOs, BAM, and Dongbo ceramics (Osteogrow-C, where C stands for ceramics) were evaluated in the rodent subcutaneous ectopic bone formation assay. Osteogrow-C device was prepared as follows: rhBMP6 was added to blood, and blood was mixed with ceramics and left to coagulate. Osteogrow-C was implanted subcutaneously in the axillary region of Sprague-Dawley rats and the outcome was analyzed 21 days following implantation using microCT, histology, morphometric analyses, and immunohistochemistry. RESULTS: Osteogrow-C implants with all tested ceramic particles induced the formation of the bone-ceramic structure containing cortical bone, the bone between the particles, and bone at the ceramic surfaces. The amount of newly formed bone was significant in all experimental groups; however, the highest bone volume was measured in Osteogrow-C implants with highly porous Vitoss ceramics. The trabecular number was highest in Osteogrow-C implants with Vitoss and ChronOs ceramics while trabeculae were thicker in implants containing BAM and Dongbo ceramics. The immunological response and inflammation were comparable among ceramic particles evaluated in this study. CONCLUSION: Osteogrow-C bone regenerative device was effective with a broad range of commercially available synthetic ceramics providing a promising therapeutic solution for the regeneration of long bone fracture nonunion, large segmental defects, and spinal fusion surgeries.
PURPOSE: Osteogrow, an osteoinductive device containing recombinant human Bone Morphogenetic Protein 6 (rhBMP6) in autologous blood coagulum, is a novel therapeutic solution for bone regeneration. This study aimed to evaluate different commercially available calcium phosphate synthetic ceramic particles as a compression-resistant matrix (CRM) added to Osteogrow implants to enhance their biomechanical properties. METHODS: Osteogrow implants with the addition of Vitoss, ChronOs, BAM, and Dongbo ceramics (Osteogrow-C, where C stands for ceramics) were evaluated in the rodent subcutaneous ectopic bone formation assay. Osteogrow-C device was prepared as follows: rhBMP6 was added to blood, and blood was mixed with ceramics and left to coagulate. Osteogrow-C was implanted subcutaneously in the axillary region of Sprague-Dawley rats and the outcome was analyzed 21 days following implantation using microCT, histology, morphometric analyses, and immunohistochemistry. RESULTS: Osteogrow-C implants with all tested ceramic particles induced the formation of the bone-ceramic structure containing cortical bone, the bone between the particles, and bone at the ceramic surfaces. The amount of newly formed bone was significant in all experimental groups; however, the highest bone volume was measured in Osteogrow-C implants with highly porous Vitoss ceramics. The trabecular number was highest in Osteogrow-C implants with Vitoss and ChronOs ceramics while trabeculae were thicker in implants containing BAM and Dongbo ceramics. The immunological response and inflammation were comparable among ceramic particles evaluated in this study. CONCLUSION: Osteogrow-C bone regenerative device was effective with a broad range of commercially available synthetic ceramics providing a promising therapeutic solution for the regeneration of long bone fracture nonunion, large segmental defects, and spinal fusion surgeries.
Authors: Lovorka Grgurevic; Hermann Oppermann; Marko Pecin; Igor Erjavec; Hrvoje Capak; Martina Pauk; Sven Karlovic; Vera Kufner; Marija Lipar; Jadranka Bubic Spoljar; Tatjana Bordukalo-Niksic; Drazen Maticic; Mihaela Peric; Reinhard Windhager; T Kuber Sampath; Slobodan Vukicevic Journal: JBMR Plus Date: 2018-11-05
Authors: Nikola Štoković; Natalia Ivanjko; Marko Pećin; Igor Erjavec; Sven Karlović; Ana Smajlović; Hrvoje Capak; Milan Milošević; Jadranka Bubić Špoljar; Dražen Vnuk; Dražen Matičić; Hermann Oppermann; T Kuber Sampath; Slobodan Vukičević Journal: Bone Date: 2020-07-27 Impact factor: 4.398
Authors: Nikola Stokovic; Natalia Ivanjko; Igor Erjavec; Milan Milosevic; Hermann Oppermann; Larry Shimp; Kuber T Sampath; Slobodan Vukicevic Journal: Bone Date: 2020-09-23 Impact factor: 4.398
Authors: Nikola Stokovic; Natalia Ivanjko; Marina Milesevic; Ivona Matic Jelic; Kristian Bakic; Viktorija Rumenovic; Hermann Oppermann; Larry Shimp; T Kuber Sampath; Marko Pecina; Slobodan Vukicevic Journal: Int Orthop Date: 2020-10-14 Impact factor: 3.075
Authors: Slobodan Vukicevic; Lovorka Grgurevic; Igor Erjavec; Marko Pecin; Tatjana Bordukalo-Niksic; Nikola Stokovic; Marija Lipar; Hrvoje Capak; Drazen Maticic; Reinhard Windhager; T Kuber Sampath; Munish Gupta Journal: J Tissue Eng Regen Med Date: 2019-11-10 Impact factor: 3.963
Authors: Catharina Chiari; Lovorka Grgurevic; Tatjana Bordukalo-Niksic; Hermann Oppermann; Alexander Valentinitsch; Elena Nemecek; Kevin Staats; Markus Schreiner; Carmen Trost; Alexander Kolb; Franz Kainberger; Sanja Pehar; Milan Milosevic; Snjezana Martinovic; Mihaela Peric; T Kuber Sampath; Slobodan Vukicevic; Reinhard Windhager Journal: J Bone Miner Res Date: 2020-07-02 Impact factor: 6.741