Marília Rizzon Zaparolli Ramos1, Ingrid Felicidade2,3, Lígia de Oliveira Carlos1, Nathalia Ramori Farinha Wagner1, Mário Sérgio Mantovani4, Luan Vitor Alves de Lima4, Lúcia Regina Ribeiro5, Thiago Inácio Barros Lopes6, Fernanda Carla Henrique-Bana7, João Vitor Zimmerman8, Fernando Cesar Macedo Junior8, Magda Rosa Ramos da Cruz9, Antônio Carlos Ligocki Campos1. 1. Department of Clinical Surgery, Federal University of Parana, Curitiba, Brazil. 2. Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, Brazil. ingridfelicidade@uel.br. 3. School of Medicine, Department of Pathology, São Paulo State University (UNESP), São Paulo, Brazil. ingridfelicidade@uel.br. 4. Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, Brazil. 5. School of Medicine, Department of Pathology, São Paulo State University (UNESP), São Paulo, Brazil. 6. Department of Chemistry, Federal University of Mato Grosso do Sul, Campo Grande, Brazil. 7. Department of Food Science and Technology, Center of Agrarian Sciences, State University of Londrina, Londrina, Brazil. 8. Department of Chemistry, Center of Exact Sciences, State University of Londrina, Londrina, Brazil. 9. Department of Nutrition, Pontifical Catholic University of Paraná (PUCPR), Curitiba, Brazil.
Abstract
BACKGROUND/ OBJECTIVE: There is evidence that metabolic profile changes after Roux-Y gastric bypass (RYGB), especially due to modifications in the gastrointestinal tract. In addition, previous studies have suggested that probiotics can modify the microbiome and produce metabolites important for metabolic health maintenance. In this sense, the aim of this study was to verify the influence of probiotic supplementation on the plasma metabolite profile after RYGB. METHODS: This was a randomized, double-blind, placebo-controlled clinical trial conducted with 31 patients subjected to RYGB surgery, randomized in probiotic group that was supplemented with a probiotic supplement (FloraVantage®) for 3 months after surgery or a placebo group. Plasma metabonomics was performed using nuclear magnetic resonance (NMR) at the preoperative period (T0) and at 45-50 days (T1) and 90-95 days (T2) during the postoperative period/intervention. RESULTS: Reductions in trimethylamine-N-oxide (TMAO) and alanine were observed in both groups, however this reduction was greater in the probiotic group (TMAO 13.82%, p = 0.01 and alanine 14.03%, p = 0.03) at T2. Additionally, β-hydroxybutyrate (BHB) levels increased 10.77% in the probiotic group (p = 0.03) compared to the placebo group at T2. CONCLUSION: Supplementation with Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 was able to associate with significant differences in relevant plasma metabolites associated with improved metabolic health.
BACKGROUND/ OBJECTIVE: There is evidence that metabolic profile changes after Roux-Y gastric bypass (RYGB), especially due to modifications in the gastrointestinal tract. In addition, previous studies have suggested that probiotics can modify the microbiome and produce metabolites important for metabolic health maintenance. In this sense, the aim of this study was to verify the influence of probiotic supplementation on the plasma metabolite profile after RYGB. METHODS: This was a randomized, double-blind, placebo-controlled clinical trial conducted with 31 patients subjected to RYGB surgery, randomized in probiotic group that was supplemented with a probiotic supplement (FloraVantage®) for 3 months after surgery or a placebo group. Plasma metabonomics was performed using nuclear magnetic resonance (NMR) at the preoperative period (T0) and at 45-50 days (T1) and 90-95 days (T2) during the postoperative period/intervention. RESULTS: Reductions in trimethylamine-N-oxide (TMAO) and alanine were observed in both groups, however this reduction was greater in the probiotic group (TMAO 13.82%, p = 0.01 and alanine 14.03%, p = 0.03) at T2. Additionally, β-hydroxybutyrate (BHB) levels increased 10.77% in the probiotic group (p = 0.03) compared to the placebo group at T2. CONCLUSION: Supplementation with Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 was able to associate with significant differences in relevant plasma metabolites associated with improved metabolic health.
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