Literature DB >> 3598193

A simple, non-chromatographic purification procedure for monoclonal antibodies. Isolation of monoclonal antibodies against cytochrome P450 isozymes.

L M Reik, S L Maines, D E Ryan, W Levin, S Bandiera, P E Thomas.   

Abstract

A simple, non-chromatographic purification procedure for monoclonal antibodies from mouse ascites fluid is described. This procedure, which is rapid, inexpensive, and has high capacity involves the precipitation of contaminating proteins with caprylic acid followed by precipitation of immunoglobulin using ammonium sulfate. This two-step procedure is shown to be effective for the purification of various immunoglobulins including IgG1, IgG2a and IgG2b. In the present report, more than 30 monoclonal antibodies directed against cytochrome P450 isozymes have been purified by this method and characterized.

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Year:  1987        PMID： 3598193     DOI： 10.1016/0022-1759(87)90180-3

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  28 in total

1.  Exploring the feasibility of an anti-idiotypic cocaine vaccine: analysis of the specificity of anticocaine antibodies (Ab1) capable of inducing Ab2beta anti-idiotypic antibodies.

Authors:  D S Schabacker; K S Kirschbaum; M Segre
Journal:  Immunology       Date:  2000-05       Impact factor: 7.397

2.  Generation of monoclonal antibodies detecting specific epitopes in olfactory and respiratory epithelia.

Authors:  J Strotmann; H Breer
Journal:  Cell Tissue Res       Date:  1991-11       Impact factor: 5.249

3.  Intratracheal administration of endotoxin and cytokines: VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation.

Authors:  T R Ulich; S C Howard; D G Remick; E S Yi; T Collins; K Guo; S Yin; J L Keene; J J Schmuke; C N Steininger
Journal:  Inflammation       Date:  1994-08       Impact factor: 4.092

4.  Restricted expression of CD2 among subsets of sheep thymocytes and T lymphocytes.

Authors:  G W Giegerich; W R Hein; M Miyasaka; G Tiefenthaler; T Hünig
Journal:  Immunology       Date:  1989-03       Impact factor: 7.397

5.  Quantification of 20-hydroxyeicosatetraenoic acid by colorimetric competitive enzyme linked immunosorbent assay.

Authors:  Harry E Grates; Richard M McGowen; Smiti V Gupta; John R Falck; Thomas R Brown; Denis M Callewaert; Diane M Sasaki
Journal:  J Biosci       Date:  2003-02       Impact factor: 1.826

6.  An Anti-Programmed Death-1 Antibody (αPD-1) Fusion Protein That Self-Assembles into a Multivalent and Functional αPD-1 Nanoparticle.

Authors:  Peng Zhao; Djordje Atanackovic; Shuyun Dong; Hideo Yagita; Xiao He; Mingnan Chen
Journal:  Mol Pharm       Date:  2017-04-19       Impact factor: 4.939

7.  Evidence from dwarf rats that growth hormone may not regulate the sexual differentiation of liver cytochrome P450 enzymes and steroid 5 alpha-reductase.

Authors:  P Bullock; B Gemzik; D Johnson; P Thomas; A Parkinson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

8.  Tissue distribution and regulation of plasminogen activator inhibitor-1 in obese mice.

Authors:  F Samad; D J Loskutoff
Journal:  Mol Med       Date:  1996-09       Impact factor: 6.354

9.  Production and Characterization of Monoclonal Antibodies against NADPH-Cytochrome P-450 Reductases from Helianthus tuberosus.

Authors:  A Lesot; I Benveniste; M P Hasenfratz; F Durst
Journal:  Plant Physiol       Date:  1992-11       Impact factor: 8.340

10.  Serological diagnosis of Brucella infections in odontocetes.

Authors:  Gabriela Hernández-Mora; Charles A Manire; Rocío González-Barrientos; Elías Barquero-Calvo; Caterina Guzmán-Verri; Lydia Staggs; Rachel Thompson; Esteban Chaves-Olarte; Edgardo Moreno
Journal:  Clin Vaccine Immunol       Date:  2009-04-22
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