Gloria Vega1, Germán Ricaurte2, Mauricio Estrada-Castrillón3,4, Harmen Reyngoudt5, Oscar M Cardona6, Jaime A Gallo-Villegas4, Raul Narvaez-Sanchez1, Juan C Calderón7. 1. Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia. 2. Group of Biophysics, University of Antioquia, Medellín, Colombia. 3. Pablo Tobón Uribe Hospital, Medellín, Colombia. 4. Group of Sports Medicine GRINMADE, Faculty of Medicine, University of Antioquia, Medellín, Colombia. 5. NMR Laboratory, Neuromuscular Investigation Center, Institute of Myology, Paris, France. 6. Indeportes Antioquia, Medellín, Colombia. 7. Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia. jcalderonv00@yahoo.com.
Abstract
OBJECTIVE: To standardize a method for 1H MRS intramuscular absolute quantification of carnosine in the thigh, using a surface coil and water as internal reference. MATERIALS AND METHODS: Carnosine spectra were acquired in phantoms (5, 10, and 15 mM) as well as in the right gastrocnemius medialis (GM) and right vastus lateralis (VLM) muscles of young team sports athletes, using volume (VC) and surface (SC) coils on a 3 T scanner, with the same receiver gain. Water spectra were used as internal reference for the absolute quantification of carnosine. RESULTS: Phantom's experiments showed a maximum error of 7%, highlighting the validity of the measurements in the study setup. The carnosine concentrations (mmol/kg ww, mean ± SD) measured in the GM were 6.8 ± 2.2 with the VC (CcarVC) and 10.2 ± 3.0 with the SC (CcarSC) (P = 0.013; n = 9). Therefore, a correction was applied to these measurements (CcarVC = 0.6582*CcarSC), to make coils performance comparable (6.8 ± 2.2 for VC and 6.7 ± 2.0 for SC, P = 0.97). After that, only the SC was used to quantify carnosine in the VLM, where a concentration of 5.4 ± 1.5 (n = 30) was found, with significant differences between men (6.2 ± 1.3; n = 15) and women (4.6 ± 1.2; n = 15). The error in quantitation was 5.3-5.5% with both coils. CONCLUSION: The method using the SC and water as internal reference can be used to quantify carnosine in voluminous muscles and regions of the body in humans, where the VC is not suitable, such as the VLM.
OBJECTIVE: To standardize a method for 1H MRS intramuscular absolute quantification of carnosine in the thigh, using a surface coil and water as internal reference. MATERIALS AND METHODS: Carnosine spectra were acquired in phantoms (5, 10, and 15 mM) as well as in the right gastrocnemius medialis (GM) and right vastus lateralis (VLM) muscles of young team sports athletes, using volume (VC) and surface (SC) coils on a 3 T scanner, with the same receiver gain. Water spectra were used as internal reference for the absolute quantification of carnosine. RESULTS: Phantom's experiments showed a maximum error of 7%, highlighting the validity of the measurements in the study setup. The carnosine concentrations (mmol/kg ww, mean ± SD) measured in the GM were 6.8 ± 2.2 with the VC (CcarVC) and 10.2 ± 3.0 with the SC (CcarSC) (P = 0.013; n = 9). Therefore, a correction was applied to these measurements (CcarVC = 0.6582*CcarSC), to make coils performance comparable (6.8 ± 2.2 for VC and 6.7 ± 2.0 for SC, P = 0.97). After that, only the SC was used to quantify carnosine in the VLM, where a concentration of 5.4 ± 1.5 (n = 30) was found, with significant differences between men (6.2 ± 1.3; n = 15) and women (4.6 ± 1.2; n = 15). The error in quantitation was 5.3-5.5% with both coils. CONCLUSION: The method using the SC and water as internal reference can be used to quantify carnosine in voluminous muscles and regions of the body in humans, where the VC is not suitable, such as the VLM.
Authors: Mahir S Ozdemir; Harmen Reyngoudt; Yves De Deene; Hakan S Sazak; Els Fieremans; Steven Delputte; Yves D'Asseler; Wim Derave; Ignace Lemahieu; Eric Achten Journal: Phys Med Biol Date: 2007-11-06 Impact factor: 3.609
Authors: Wim Derave; Mahir S Ozdemir; Roger C Harris; Andries Pottier; Harmen Reyngoudt; Katrien Koppo; John A Wise; Eric Achten Journal: J Appl Physiol (1985) Date: 2007-08-09
Authors: Barbora de Courten; Timea Kurdiova; Maximilian P J de Courten; Vitazoslav Belan; Inge Everaert; Marek Vician; Helena Teede; Daniela Gasperikova; Giancarlo Aldini; Wim Derave; Jozef Ukropec; Barbara Ukropcova Journal: PLoS One Date: 2015-10-06 Impact factor: 3.240