| Literature DB >> 359711 |
J H Berden, J F Hagemann, R A Koene.
Abstract
Under appropriate conditions the haemolytic activity of mouse complement can be measured in a 51Cr release assay. Modifications that increase the sensitivity are the following: (a) mouse 7 S anti-SRBC antibodies as amboceptor; (b) low amount of target cells; (c) ionic strength of 0.13 M NaCl in the test medium; (d) incubation temperature of 30 degrees C; (e) incubation time of 90 min; (f) total reaction volume of 1 ml.Entities:
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Year: 1978 PMID： 359711 DOI： 10.1016/0022-1759(78)90118-7
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303