Kuaiyun Yu1, Heng Zhu2. 1. Department of General Surgery, Yantaishan Hospital Yantai 264001, Shandong, China. 2. Department of Digestive, The Fourth People's Hospital of Ji'nan Ji'nan 250031, Shandong, China.
Abstract
OBJECTIVE: MiR-762 has been confirmed as a tumor promoter in multiple tumors, while few reports illustrate its role in gastric cancer (GC). Thus, this research aimed to investigate whether miR-762 is involved in GC development. METHODS: MiR-762 expression in the tumor tissues from GC patients and GC cell lines was analyzed by qRT-PCR. The assays including CCK-8, transwell, and flow cytometry were performed to reveal the functions of miR-762 in GC. The target genes of miR-762 were searched by online databases, and then were verified by dual-luciferase reporter assay. Western blot was performed to investigate the activation of PI3K/AKT and Hippo pathways in GC. RESULTS: MiR-762 was aberrantly upregulated in the tumor tissues and cell lines, and miR-762 silencing could effectively reduce the viability and promote apoptosis of GC cell lines. The study identified LZTS1 as a target gene of miR-762. It was also found that the effects of miR-762 on GC cells could be reversed by LZTS1, and miR-762 could upregulate the activation of the PI3K/AKT pathway but inhibit the Hippo pathway by targeting LZTS1. CONCLUSION: MiR-762 activates PI3K/AKT and suppresses Hippo pathways to boost GC proliferation and invasion by targeting LZTS1. AJTR
OBJECTIVE: MiR-762 has been confirmed as a tumor promoter in multiple tumors, while few reports illustrate its role in gastric cancer (GC). Thus, this research aimed to investigate whether miR-762 is involved in GC development. METHODS: MiR-762 expression in the tumor tissues from GC patients and GC cell lines was analyzed by qRT-PCR. The assays including CCK-8, transwell, and flow cytometry were performed to reveal the functions of miR-762 in GC. The target genes of miR-762 were searched by online databases, and then were verified by dual-luciferase reporter assay. Western blot was performed to investigate the activation of PI3K/AKT and Hippo pathways in GC. RESULTS: MiR-762 was aberrantly upregulated in the tumor tissues and cell lines, and miR-762 silencing could effectively reduce the viability and promote apoptosis of GC cell lines. The study identified LZTS1 as a target gene of miR-762. It was also found that the effects of miR-762 on GC cells could be reversed by LZTS1, and miR-762 could upregulate the activation of the PI3K/AKT pathway but inhibit the Hippo pathway by targeting LZTS1. CONCLUSION: MiR-762 activates PI3K/AKT and suppresses Hippo pathways to boost GC proliferation and invasion by targeting LZTS1. AJTR
Authors: Beate Rau; Andreas Brandl; Pompiliu Piso; Jörg Pelz; Peter Busch; Cedric Demtröder; Silke Schüle; Hans-Jürgen Schlitt; Marc Roitman; Jürgen Tepel; Udo Sulkowski; Faik Uzunoglu; Michael Hünerbein; Rüdiger Hörbelt; Michael Ströhlein; Stefan Beckert; Ingmar Königsrainer; Alfred Königsrainer Journal: Gastric Cancer Date: 2019-06-21 Impact factor: 7.370
Authors: Qiao Guanen; Shi Junjie; Wu Baolin; Wang Chaoyang; Yang Yajuan; Li Jing; Li Junpeng; Ning Gaili; Wang Zhongping; Wang Jun Journal: Biomed Pharmacother Date: 2018-06-01 Impact factor: 6.529