Shashikumara Shashikumara1, Krishna Purushotham1, C L Darshan1, Bhuvanesh Sukhlal Kalal2. 1. Department of Pharmacology, Chamarajanagar Institute of Medical Sciences Chamarajanagar 571313, Karnataka, India. 2. Department of Pharmacology and Nutritional Sciences, University of Kentucky, College of Medicine Lexington Kentucky 40536, USA.
Abstract
BACKGROUND: Long-term stress and chronic stress events play an important role in the etiology of depression. The study aimed to investigate the antidepressant-like effect of freshly prepared crude ethanolic extract of Saraca asoca flower (ESAF) in a mice model of acute restraint stress. METHODOLOGY: Rhamnazin, Myricetin and Quercetin were analytically characterized through liquid chromatography-mass spectrometry and high-performance liquid chromatography from Saraca asoca flower in a 0.1% acetic acid fraction of ethanol. The antidepressant effect was tested by repeated administration of freshly prepared ESAF on mice subjected to repeated and different forms of stress induction for 2 hours every day in the morning and night for seven consecutive days. The antidepressant activity was measured by known behavioral animal models: forced swim test (FST) and tail suspension test (TST). At the end of the experiment, each group of mice was sacrificed by cervical dislocation, followed by an estimation of the biochemical data. RESULTS: The oral administration of ESAF in doses of 50, 100, & 250 mg/kg for seven consecutive days gave a significant decrease in the time of immobility (P<0.05) and reversed the depression-like behavior induced by acute restraint methods and behavioral models. ESAF treated groups showed a significant increase in glutathione peroxidase (GSH-PX) activity in the hippocampus of the acutely restrained mice. In addition, ESAF 250 mg/kg reduced plasma corticosterone levels in mice subjected to different forms of acute restraint stress compared to other groups, comparable to the standard imipramine. CONCLUSION: Our study showed the antidepressant activity of the ESAF. This effect may be attributed to the presence of antioxidant bioflavonoids namely, Rhamnazin, Myricetin and quercetin. Reduction in the plasma corticosterone levels along with an increase in the antioxidant enzymatic activity such as GSP-PX and SOD in the mice's hippocampus is the proposed molecular hypothesis for its neuroprotective mechanism. AJTR
BACKGROUND: Long-term stress and chronic stress events play an important role in the etiology of depression. The study aimed to investigate the antidepressant-like effect of freshly prepared crude ethanolic extract of Saraca asoca flower (ESAF) in a mice model of acute restraint stress. METHODOLOGY: Rhamnazin, Myricetin and Quercetin were analytically characterized through liquid chromatography-mass spectrometry and high-performance liquid chromatography from Saraca asoca flower in a 0.1% acetic acid fraction of ethanol. The antidepressant effect was tested by repeated administration of freshly prepared ESAF on mice subjected to repeated and different forms of stress induction for 2 hours every day in the morning and night for seven consecutive days. The antidepressant activity was measured by known behavioral animal models: forced swim test (FST) and tail suspension test (TST). At the end of the experiment, each group of mice was sacrificed by cervical dislocation, followed by an estimation of the biochemical data. RESULTS: The oral administration of ESAF in doses of 50, 100, & 250 mg/kg for seven consecutive days gave a significant decrease in the time of immobility (P<0.05) and reversed the depression-like behavior induced by acute restraint methods and behavioral models. ESAF treated groups showed a significant increase in glutathione peroxidase (GSH-PX) activity in the hippocampus of the acutely restrained mice. In addition, ESAF 250 mg/kg reduced plasma corticosterone levels in mice subjected to different forms of acute restraint stress compared to other groups, comparable to the standard imipramine. CONCLUSION: Our study showed the antidepressant activity of the ESAF. This effect may be attributed to the presence of antioxidant bioflavonoids namely, Rhamnazin, Myricetin and quercetin. Reduction in the plasma corticosterone levels along with an increase in the antioxidant enzymatic activity such as GSP-PX and SOD in the mice's hippocampus is the proposed molecular hypothesis for its neuroprotective mechanism. AJTR