Purpose: Understanding the humoral immune response dynamics carried out by B cells in COVID-19 vaccination is little explored; therefore, we analyze the changes induced in the different cellular subpopulations of B cells after vaccination with BNT162b2 (Pfizer-BioNTech). Methods: This prospective cohort study evaluated thirty-nine immunized health workers (22 with prior COVID-19 and 17 without prior COVID-19) and ten subjects not vaccinated against SARS-CoV-2 (control group). B cell subpopulations (transitional, mature, naïve, memory, plasmablasts, early plasmablast, and double-negative B cells) and neutralizing antibody levels were analyzed and quantified by flow cytometry and ELISA, respectively. Results: The dynamics of the B cells subpopulations after vaccination showed the following pattern: the percentage of transitional B cells was higher in the prior COVID-19 group (p < 0.05), whereas virgin B cells were more prevalent in the group without prior COVID-19 (p < 0.05), mature B cells predominated in both vaccinated groups (p < 0.01), and memory B cells, plasmablasts, early plasmablasts, and double-negative B cells were higher in the not vaccinated group (p < 0.05). Conclusion: BNT162b2 vaccine induces changes in B cell subpopulations, especially generating plasma cells and producing neutralizing antibodies against SARS-CoV-2. However, the previous infection with SARS-CoV-2 does not significantly alter the dynamics of these subpopulations but induces more rapid and optimal antibody production.
Purpose: Understanding the humoral immune response dynamics carried out by B cells in COVID-19 vaccination is little explored; therefore, we analyze the changes induced in the different cellular subpopulations of B cells after vaccination with BNT162b2 (Pfizer-BioNTech). Methods: This prospective cohort study evaluated thirty-nine immunized health workers (22 with prior COVID-19 and 17 without prior COVID-19) and ten subjects not vaccinated against SARS-CoV-2 (control group). B cell subpopulations (transitional, mature, naïve, memory, plasmablasts, early plasmablast, and double-negative B cells) and neutralizing antibody levels were analyzed and quantified by flow cytometry and ELISA, respectively. Results: The dynamics of the B cells subpopulations after vaccination showed the following pattern: the percentage of transitional B cells was higher in the prior COVID-19 group (p < 0.05), whereas virgin B cells were more prevalent in the group without prior COVID-19 (p < 0.05), mature B cells predominated in both vaccinated groups (p < 0.01), and memory B cells, plasmablasts, early plasmablasts, and double-negative B cells were higher in the not vaccinated group (p < 0.05). Conclusion: BNT162b2 vaccine induces changes in B cell subpopulations, especially generating plasma cells and producing neutralizing antibodies against SARS-CoV-2. However, the previous infection with SARS-CoV-2 does not significantly alter the dynamics of these subpopulations but induces more rapid and optimal antibody production.
Authors: Swetha Garimalla; Doan C Nguyen; Jessica L Halliley; Christopher Tipton; Alexander F Rosenberg; Christopher F Fucile; Celia L Saney; Shuya Kyu; Denise Kaminski; Yu Qian; Richard H Scheuermann; Greg Gibson; Iñaki Sanz; F Eun-Hyung Lee Journal: JCI Insight Date: 2019-05-02
Authors: Christoph Ruschil; Gisela Gabernet; Gildas Lepennetier; Simon Heumos; Miriam Kaminski; Zsuzsanna Hracsko; Martin Irmler; Johannes Beckers; Ulf Ziemann; Sven Nahnsen; Gregory P Owens; Jeffrey L Bennett; Bernhard Hemmer; Markus C Kowarik Journal: Front Immunol Date: 2020-12-18 Impact factor: 7.561
Authors: Victoria Hall; Sarah Foulkes; Ferdinando Insalata; Peter Kirwan; Ayoub Saei; Ana Atti; Edgar Wellington; Jameel Khawam; Katie Munro; Michelle Cole; Caio Tranquillini; Andrew Taylor-Kerr; Nipunadi Hettiarachchi; Davina Calbraith; Noshin Sajedi; Iain Milligan; Yrene Themistocleous; Diane Corrigan; Lisa Cromey; Lesley Price; Sally Stewart; Elen de Lacy; Chris Norman; Ezra Linley; Ashley D Otter; Amanda Semper; Jacqueline Hewson; Silvia D'Arcangelo; Meera Chand; Colin S Brown; Tim Brooks; Jasmin Islam; Andre Charlett; Susan Hopkins Journal: N Engl J Med Date: 2022-02-16 Impact factor: 91.245