Literature DB >> 3595736

A method for freezing synchronous mitotic and G1 cells.

M J Borrelli, M A Mackey, W C Dewey.   

Abstract

A modification of the protocol developed by Kawamoto, J C & Barrett, J N, Brain res (1986), in press for freezing primary neuron cultures in a solution containing low sodium and high lactate and potassium concentrations was used to freeze synchronous mitotic and G1 CHO cells. After thawing, the cells behaved as if they had never been frozen with respect to cell growth, cell division, plating efficiency, and hyperthermic sensitivity.

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Year:  1987        PMID: 3595736     DOI: 10.1016/0014-4827(87)90313-2

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN: 0014-4827            Impact factor:   3.905


  5 in total

1.  Transcription initiation from the dihydrofolate reductase promoter is positioned by HIP1 binding at the initiation site.

Authors:  A L Means; P J Farnham
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

2.  Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain.

Authors:  C J Fry; J E Slansky; P J Farnham
Journal:  Mol Cell Biol       Date:  1997-04       Impact factor: 4.272

3.  Sequences downstream of the transcription initiation site modulate the activity of the murine dihydrofolate reductase promoter.

Authors:  P J Farnham; A L Means
Journal:  Mol Cell Biol       Date:  1990-04       Impact factor: 4.272

4.  The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II.

Authors:  A B Buermeyer; N E Thompson; L A Strasheim; R R Burgess; P J Farnham
Journal:  Mol Cell Biol       Date:  1992-05       Impact factor: 4.272

5.  The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter.

Authors:  A L Means; J E Slansky; S L McMahon; M W Knuth; P J Farnham
Journal:  Mol Cell Biol       Date:  1992-03       Impact factor: 4.272

  5 in total

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