| Literature DB >> 35951196 |
Sylvia Rodrigues da Silveira1, Andréa Dias Koehler1, Ana Cristina M Mendes Gomes2, Glaucia Barbosa Cabral2, Vera Tavares de Campos Carneiro2, Diva Maria de Alencar Dusi3, Adriana Pinheiro Martinelli4.
Abstract
In situ hybridization with mRNA probes enables the detection and localization of gene expression in plant somatic embryogenesis samples. BbrizSERK is a gene that is expressed in embryogenic cells and tissues of Brachiaria. Here we describe methods used for in situ hybridization to localize BbrizSERK transcripts during somatic embryogenesis of Brachiaria brizantha according to the plant material and observations intended, using paraffin or butyl methyl methacrylate resin-embedded samples, as well as a method for whole-mount preparation applicable for the analysis of other genes involved in embryogenic processes, along with other in vitro processes.Entities:
Keywords: Brachiaria; Butyl methyl methacrylate; Gene expression; Histological sections; Paraffin; SERK; Whole mount; mRNA probes
Mesh：
Year: 2022 PMID： 35951196 DOI： 10.1007/978-1-0716-2485-2_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745