Literature DB >> 3594583

Optimum conditions for electric pulse-mediated gene transfer to mammalian cells in suspension.

H Hama-Inaba, M Takahashi, M Kasai, T Shiomi, A Ito, F Hanaoka, K Sato.   

Abstract

A pulse-generating machine which delivers exponentially decaying pulses over broad range of pulse lengths was used to determine the optimum pulse conditions for gene transfer to FM3A cells. In the transformation of tk- cells with pTK1, a single pulse of 100-2000 microseconds gave a high transformation frequency at 1.5-6 kV/cm and room temperature, the highest transformation frequency obtained being 3 X 10(-3). As the suspension buffer for cells exposed to the pulse, Saline G was better than PBS(-) for obtaining a large number of transformants because it ensured high cell viability.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3594583     DOI： 10.1247/csf.12.173

Source DB:  PubMed          Journal:  Cell Struct Funct        ISSN： 0386-7196            Impact factor:   2.212


  7 in total

Review 1.  Improvement of DNA transfection with cationic liposomes.

Authors:  A Rocha; S Ruiz; J M Coll
Journal:  J Physiol Biochem       Date:  2002-03       Impact factor: 4.158

2.  Model of creation and evolution of stable electropores for DNA delivery.

Authors:  Kyle C Smith; John C Neu; Wanda Krassowska
Journal:  Biophys J       Date:  2004-05       Impact factor: 4.033

3.  Modeling electroporation in a single cell.

Authors:  Wanda Krassowska; Petar D Filev
Journal:  Biophys J       Date:  2006-10-20       Impact factor: 4.033

4.  End extension repair of introduced targeting vectors mediated by homologous recombination in mammalian cells.

Authors:  Y Aratani; R Okazaki; H Koyama
Journal:  Nucleic Acids Res       Date:  1992-09-25       Impact factor: 16.971

5.  Molecular identification of a human carcinoma-associated glycoprotein antigen recognized by mouse monoclonal antibody FU-MK-1.

Authors:  Y Tomita; F Arakawa; T Yamamoto; M Kuwahara; R Watanabe; H Iwasaki; M Kikuchi; M Kuroki
Journal:  Jpn J Cancer Res       Date:  2000-02

6.  Metabolism and toxicity of electroporated 1-beta-D-arabinofuranosylcytosine triphosphate in a human leukemia cell line.

Authors:  M Kubota; T Yorifuji; H Hashimoto; T Shimizu; H Mikawa
Journal:  Jpn J Cancer Res       Date:  1990-12

Review 7.  Does the shape of the electric pulse matter in electroporation?

Authors:  Vitalij Novickij; Nina Rembiałkowska; Wojciech Szlasa; Julita Kulbacka
Journal:  Front Oncol       Date:  2022-09-14       Impact factor: 5.738
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.