Jason G van Genderen1, Cecilia Chia2, Malon Van den Hof2, Henk J M M Mutsaerts3, Liesbeth Reneman4, Dasja Pajkrt2, Anouk Schrantee4. 1. Department of Pediatric Infectious Diseases, Emma Children's Hospital, Amsterdam UMC, location Academic Medical Center, Amsterdam, the Netherlands j.g.vangenderen@amsterdamumc.nl. 2. Department of Pediatric Infectious Diseases, Emma Children's Hospital, Amsterdam UMC, location Academic Medical Center, Amsterdam, the Netherlands. 3. Department of Radiology and Nuclear Medicine, Amsterdam University Medical Centers, location VU Medical Center, University of Amsterdam, Amsterdam, the Netherlands. 4. Department of Radiology and Nuclear Medicine, Amsterdam University Medical Centers, location Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
Abstract
BACKGROUND AND OBJECTIVES: Despite effective combination antiretroviral therapy (cART), adolescents with perinatally acquired HIV (PHIV) exhibit cognitive impairment, of which structural changes could be the underlying pathophysiological mechanism. Prior MRI studies found lower brain volumes, more white matter (WM) hyperintensities (WMH) volume, lower WM integrity, and differences in cerebral blood flow (CBF). However, these findings may be confounded by adoption status, as the large portion PHIV adolescents have been adopted. Adoption has been associated with malnutrition and neglect which in turn may have affected brain development. We investigated the long-term effects of PHIV on the brain, while minimizing the confounding effect of adoption status. METHODS: We determined whole brain gray matter (GM) and WM volume with 3D T1-weighted scans; total WMH volume with fluid-attenuated inversion recovery (FLAIR); CBF in the following regions of interest (ROIs): WM, GM and subcortical GM with arterial spin labeling (ASL); and whole brain WM microstructural markers: fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), radial diffusivity (RD) with diffusion tensor imaging (DTI) in cART treated PHIV adolescents visiting our outpatient clinic in Amsterdam and controls matched for age, sex, ethnic origin, socio-economic status, and adoption status. We assessed differences in neuroimaging parameters between PHIV adolescents and controls using linear regression models adjusted for age and sex and applied multiple comparisons correction. RESULTS: 35 PHIV adolescents and 38 controls were included with a median age (years) of 14.9 (IQR: 10.7-18.5) and 15.6 (IQR:11.1-17.6), respectively with a similar rate of adoption. We found a lower overall FA (beta = -0.012; p<0.014, -2.4%), higher MD (beta = 0.014, p = 0.014, 1.3%) and higher RD (beta = 0.02, p = 0.014, 3.3%) in PHIV adolescents vs. adoption-matched controls, but no differences in AD. We found comparable GM, WM and WMH volume, and CBF in ROIs between PHIV adolescents and controls. We did not find an association between cognitive profiles and WM microstructural markers in PHIV adolescents. DISCUSSION: Irrespective of adoption status, PHIV adolescents exhibited subtle lower WM integrity. Our findings may point towards early-acquired WM microstructural alterations associated with HIV.
BACKGROUND AND OBJECTIVES: Despite effective combination antiretroviral therapy (cART), adolescents with perinatally acquired HIV (PHIV) exhibit cognitive impairment, of which structural changes could be the underlying pathophysiological mechanism. Prior MRI studies found lower brain volumes, more white matter (WM) hyperintensities (WMH) volume, lower WM integrity, and differences in cerebral blood flow (CBF). However, these findings may be confounded by adoption status, as the large portion PHIV adolescents have been adopted. Adoption has been associated with malnutrition and neglect which in turn may have affected brain development. We investigated the long-term effects of PHIV on the brain, while minimizing the confounding effect of adoption status. METHODS: We determined whole brain gray matter (GM) and WM volume with 3D T1-weighted scans; total WMH volume with fluid-attenuated inversion recovery (FLAIR); CBF in the following regions of interest (ROIs): WM, GM and subcortical GM with arterial spin labeling (ASL); and whole brain WM microstructural markers: fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), radial diffusivity (RD) with diffusion tensor imaging (DTI) in cART treated PHIV adolescents visiting our outpatient clinic in Amsterdam and controls matched for age, sex, ethnic origin, socio-economic status, and adoption status. We assessed differences in neuroimaging parameters between PHIV adolescents and controls using linear regression models adjusted for age and sex and applied multiple comparisons correction. RESULTS: 35 PHIV adolescents and 38 controls were included with a median age (years) of 14.9 (IQR: 10.7-18.5) and 15.6 (IQR:11.1-17.6), respectively with a similar rate of adoption. We found a lower overall FA (beta = -0.012; p<0.014, -2.4%), higher MD (beta = 0.014, p = 0.014, 1.3%) and higher RD (beta = 0.02, p = 0.014, 3.3%) in PHIV adolescents vs. adoption-matched controls, but no differences in AD. We found comparable GM, WM and WMH volume, and CBF in ROIs between PHIV adolescents and controls. We did not find an association between cognitive profiles and WM microstructural markers in PHIV adolescents. DISCUSSION: Irrespective of adoption status, PHIV adolescents exhibited subtle lower WM integrity. Our findings may point towards early-acquired WM microstructural alterations associated with HIV.
Authors: Henk J M M Mutsaerts; Jan Petr; Paul Groot; Pieter Vandemaele; Silvia Ingala; Andrew D Robertson; Lena Václavů; Inge Groote; Hugo Kuijf; Fernando Zelaya; Owen O'Daly; Saima Hilal; Alle Meije Wink; Ilse Kant; Matthan W A Caan; Catherine Morgan; Jeroen de Bresser; Elisabeth Lysvik; Anouk Schrantee; Astrid Bjørnebekk; Patricia Clement; Zahra Shirzadi; Joost P A Kuijer; Viktor Wottschel; Udunna C Anazodo; Dasja Pajkrt; Edo Richard; Reinoud P H Bokkers; Liesbeth Reneman; Mario Masellis; Matthias Günther; Bradley J MacIntosh; Eric Achten; Michael A Chappell; Matthias J P van Osch; Xavier Golay; David L Thomas; Enrico De Vita; Atle Bjørnerud; Aart Nederveen; Jeroen Hendrikse; Iris Asllani; Frederik Barkhof Journal: Neuroimage Date: 2020-06-08 Impact factor: 6.556
Authors: Jacqueline Hoare; Jean-Paul Fouche; Nicole Phillips; John A Joska; Robert Paul; Kirsten A Donald; Kevin G F Thomas; Dan J Stein Journal: AIDS Date: 2015-09-10 Impact factor: 4.177
Authors: Robert H Paul; Sarah Phillips; Jacqueline Hoare; David H Laidlaw; Ryan Cabeen; Gayla R Olbricht; Yuqing Su; Dan J Stein; Susan Engelbrecht; Soraya Seedat; Lauren E Salminen; Laurie M Baker; Jodi Heaps; John Joska Journal: J Neurovirol Date: 2016-12-02 Impact factor: 2.643
Authors: Malon Van den Hof; Pien E J Jellema; Anne Marleen Ter Haar; Henriette J Scherpbier; Anouk Schrantee; Antonia Kaiser; Matthan W A Caan; Charles B L M Majoie; Peter Reiss; Ferdinand W N M Wit; Henk-Jan M M Mutsaerts; Dasja Pajkrt Journal: AIDS Date: 2021-07-01 Impact factor: 4.632
Authors: Charlotte Blokhuis; Henri J M M Mutsaerts; Sophie Cohen; Henriëtte J Scherpbier; Matthan W A Caan; Charles B L M Majoie; Taco W Kuijpers; Peter Reiss; Ferdinand W N M Wit; Dasja Pajkrt Journal: Medicine (Baltimore) Date: 2017-02 Impact factor: 1.889